摘要
目的探讨FGF-2与TGF-β1单独及联合诱导对骨髓间充质干细胞(BMSCs)向心肌样细胞分化的影响。方法体外分离培养SD大鼠BMSCs,流式细胞术鉴定其表面抗原。分组诱导:A组(FGF-2)、B组(TGF-β1)、C组(FGF-2+TGF-β1),D组(空白对照)。镜下观察细胞形态改变;免疫细胞化学染色法检测Desmin、α-sarcomeric actin、cTnI的表达;实时荧光定量PCR法检测GATA-4、Nkx2.5的表达。结果 BMSCs表面抗原CD90、CD29、CD45的阳性率分别为84.7%、86.5%、0.3%。诱导后,C组细胞以梭形及类长柱形为主,出现类肌管样结构;蛋白的阳性细胞数最多(P<0.05);GATA-4、Nkx2.5的表达量高于其余两组(P<0.05)。结论 FGF-2与TGF-β1均可将BMSCs诱导为心肌样细胞,但两者联合诱导效果更佳。
Objective To expore the effects of FGF-2 and TGF-β1 on the differentiation of rat bone mar-row mesenchymal stem cells（BMSCs）towards cardiocytes. Methods BMSCs were isolated from bones of SD rats and cultured and identified by flow cytometry.The cells were divided into four groups：group A（FGF-2）,group B （TGF-β1）,group C（FGF-2＋TGF-β1）and group D（blank control）.The morphological changes were observed by the microscope. The expressions of Desmin,α-sarcomeric actin and cTnI were detected by immunocytochemical stainning.The expressions of GATA-4 and Nkx2.5was detected by RT-qPCR.Result The positive expression rate of CD90,CD29 and CD45 was 84.7%,86.5%,0.3% respectively. After induction,group C presence of myotube structures and the shape is fusiform.The positive rate of combined induction group was higher than the others （P 〈 0.05）. The expression of GATA-4 and Nkx2.5 genes was higher than that in the other groups（P 〈 0.05）. Conclution FGF-2 and TGF-β1 can be used to induce BMSCs into cardiocytes,and the combined group has the better effect.
出处
《实用医学杂志》
CAS
北大核心
2017年第24期4019-4023,共5页
The Journal of Practical Medicine
基金
河北省自然科学基金(编号:NO.H2015405017)
河北省中医药管理局科研计划项目(编号:NO.2014202)
河北省教育厅科技研究重点项目(编号:NO.ZH2012001)
河北北方学院创新人才培育项目(编号:NO.CXRC1315)
作者简介
通信作者：王海萍E-mail：haipingmimi@126．com
