摘要
目的:从深海海泥中筛选得到产酯酶的微生物,以丰富可酿酒微生物菌群和可酿酒微生物来源.方法:通过18srDNA生物学鉴定微生物种属,并以乙醇和己酸为底物,气相色谱法测定该菌株酯化合成己酸乙酯的能力。结果:从深海泥中筛选到产酯酶酵母菌1株,经18SrDNA生物学鉴定,其与近平滑假丝酵母(Candida parapsilosis)同源性大于99%。讨论:酵母有较佳的酯化能力,酯化合成己酸乙酯的含量经气相色谱检测为67.44mg/100mL,酯化力2.6976U,该海洋产酯酶酵母菌株在酯化反应中有较广泛的底物选择性,尤其对己酸有较高的选择性。
Objective： some strains producing esterase was screened from the sea mud to enrich brewed microorganisms and the source of brewage microbes. Methods： A microbial strain was identified by 18 SrDNA. By using alcohol and hexanoic acid as substrate, The strain＇s ability of esterificating ethyl caproate was detected by gas chromatography. Results： one yeast producting esterase was screened from sea mud, By 18 SrDNA biological identified the strain, which is more than 99% homologous with Candida parapsilosis in the experiment.Discussion： the yeast strain has better esterification ability. the content of ethyl caproate is 67.44 mg/100 mL. the content of esterifying capacity was 2.6976 U. The marine yeast strain has a broad substrate selectivity in esterification reaction,especially,it has higher selectivity for caproic acid.
出处
《酿酒》
CAS
2017年第6期36-42,共7页
Liquor Making
关键词
酯酶
产酯微生物
己酸乙酯
气相色谱(GC)
酯化力
Esterase
Ester-producing microbes
ethyl caproate
gas chromatography（GC）
E estenfied capability
作者简介
梁龙元（1992-），男，贵州遵义人，硕士研究生在读。;通讯作者：汪江波（1962-），男，硕士，副教授。
