摘要
目前基于大豆共生结瘤QTL定位的结果少、置信区间大,难以应用于实践中,因此整合前人研究结果、缩短置信区间具有重要意义。本研究针对大豆共生结瘤相关性状,搜集国内外已报道的48个QTL定位结果,利用Meta分析得到2个分别控制结瘤数目、结瘤大小和结瘤干鲜重的Meta-QTL。在区间内选择候选基因,利用野生大豆和栽培大豆验证候选基因在接种根瘤菌后的表达模式,明确候选基因与共生结瘤的关系。对Meta-QTL区间内的6个候选基因进行qRT-PCR检测及初步生物信息学分析,结果表明其中4个基因有可能为大豆共生结瘤性状的候选基因。本研究的实验方法对QTL定位区间内候选基因的确定和功能研究具有指导意义。
Symbiotic nodulation in soybean is an important agronomic trait affecting soybean biological nitrogen fixation. Few QTLs for symbiotic nodulation with small confidence interval could be applied in practice. Our pur-pose was to integrate the results of previous studies and propose meta - QTL. Meta - analysis was carried out based on 48 QTLs about symbiotic nodule - related traits across 4 independent studies, and two meta - QTLs controlling nodule number, nodule size and nodule dry - weight were proposed. Six candidate genes were screened from the in-terval of meta - QTL and their expressional patterns were analyzed by qRT - PCR in cultivar soybean and wild soy-bean inoculated with rhizobia. Among them, 4 genes may be involved in symbiotic nodulation in soybean. This two meta - QTLs should prove helpful in identification and functionality study of candidate genes associated with symbi-otic nodule - related traits.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2017年第5期580-588,共9页
Chinese Journal of Oil Crop Sciences
基金
国家现代农业产业技术体系(CARS-04-01A)
国家自然科学基金(31471516)
黑龙江省高校长江学者后备支持计划项目(2014CJHB004)
作者简介
张艳娇（1993-）,女,黑龙江人,硕士研究生,主要从事大豆与微生物互作分子机制研究,E-mail：13796669423@163.com
通讯作者：辛大伟（1981-）,男,黑龙江人,副研究员,硕士生导师,研究方向为大豆与微生物分子互作机制,E-mail：dwxin@neauedu.com
通讯作者：陈庆山（1973-）,男,黑龙江人,教授,博士生导师,研究方向为大豆生物技术,E-mail：qingshanchen@126.com
