摘要
为了解云南省部分地区规模化猪场种公猪精液繁殖障碍病毒性病原的感染状况,应用PCR对2014年—2016年间云南省部分地区规模化猪场212份种公猪精液进行了猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)、猪伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV2)和猪细小病毒(PPV)5种与猪繁殖障碍有关的病原检测。结果表明,PPRSV阳性率为7.08%,CSFV阳性率为3.77%,PCV2阳性率为6.60%,PRV阳性率为3.30%,没有检测出PPV,PRRSV和PCV2的感染率呈上升趋势,其他疫病保持相对平稳态势。所有的混合感染样品中,均检测出了PRRSV,其中PRRSV和PCV2的混合感染最为常见。结果提示,控制猪场疫病发生的关键是控制PRRSV和PCV2的流行,有必要加强对种猪群的疫病病毒检测,推行种猪场主要疫病的控制与净化工作。
In order to investigate the viral infection situations in some areas in Yunnan province, the detec-tion of five production related viruses from 212 boar semen by polymerase chain reaction （PCR） was per-formed, including porcine repeproductive and respiratory syndrome virus （PRRSV） , classic swine fever vi-rus （CSFV） , pseudorabies virus （PRV） , porcine circovirus type virus H （PCV2） and porcine parvovirus （PPV）. The results showed that the PPRSV positive rate is 7. 08 % ; the CSFV positive rate is 3 . 77 %; the PCV2 positive rate is 6. 60% and the PRV positive rate is 3. 30 % , while no PPV positive semen was detec-ted. The rates of PRRSV and PCV infection increased year by year,and the infection rates for the other two viruses have been relatively stable. The PRRSV were detected from all the multiplex infection samples. A-mong them,the mixed infection of PRRSV and PCV2 is most common. Our data implied that controlling PRRSV and PCV2 is the key to prevent swine diseases. It is necessary to strengthen the disease detection for breeding herd and to purify the main breeding pig farm.
出处
《动物医学进展》
北大核心
2017年第9期37-42,共6页
Progress In Veterinary Medicine
基金
云南省应用基础研究重点项目(2016FA018)
国家自然科学基金项目(31560705)
关键词
种公猪
精液
繁殖障碍
病原调查
boar
semen reproductive disorder
pathogenic investigation
作者简介
赵谦（1992-）,男,云南大理人,硕士研究生,主要从事动物传染病及分子流行病学研究
通讯作者
