摘要
本试验在四川省7个市的部分猪场采集公猪精液样本共67份,用RT-PCR方法检测出有4份CFSV抗原呈阳性,对该4株CSFV的E2基因进行克隆、测序,然后运用DNAStar序列分析软件与已知的15株CSFV E2基因进行同源性比对。
In this study, we had chosen some farms of 7 cities in Sichuan province as the researched object, 67 copies of boar＇s semen samples were collected, and 4 copies of positive CFSV antigen were detected by RT-PCR method. The E2 gene of the 4 strains＇ CSFV were cloned and sequenced. DNAStar gene sequence analysis software was used to compare homology between the detected 4 CSFV strains and the known 15 CSFV strains.
出处
《四川畜牧兽医》
2013年第10期33-34,37,共3页
Sichuan Animal & Veterinary Sciences
基金
四川省科技支撑计划项目(2012NZ0001)
关键词
猪瘟病毒
精液
分子流行病学
Classical swine fever virus（CSFV）
Semen
Molecular epidemiology
作者简介
李海全（1984-），男，四川仪陇人，研究生，初级兽医师，研究方向：动物传染病病原分子生物学．
