摘要
利用实验室筛选得到的一株枯草芽孢杆菌发酵生产γ-聚谷氨酸(γ-PGA),主要对其发酵工艺参数进行了优化。先通过单因素优化及PB试验筛选重要因子,然后利用Box-Behnken优化发酵工艺最佳组合,获得了最佳培养基组成和最佳培养条件,且在摇床水平上使γ-PGA产量由最初的6.03 g·L^(-1)提高到10.98 g·L^(-1)。接着通过对补料工艺的探索,确定了最佳补料时间和补料配方,最后在5 L发酵罐上进行了放大生产,最终γ-PGA的最高产量可达到31.18 g·L^(-1)。
In this study, we optimized the fermentation parameters of poly-y-glutamic acid produced by Ba- cillus subtilis, a strain selected through a lab screen. A single factor experiment and PB experiment were first car- ried out, and then the optimal composition of the medium and optimum culture conditions were obtained by opti- mizing the fermentation process with Box-Behnken. The yield of y-PGA increased from 6.03 g·L-1 to 10.98 g·L-1 at the shaker level. We further explored the feeding process to determine the best time and feeding materials. Fi- nally, a 5 L fermentor was used to increase the production, and the highest yield of y-PGA reached to 31.18 g· L-1.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2017年第4期567-573,共7页
Journal of Anhui Agricultural University
基金
江苏省科技厅产学研联合创新资金"前瞻性研究项目(BY2013001-03)"资助
关键词
枯草芽孢杆菌
Γ-PGA
发酵工艺优化
Bacillus subtilis
y - PGA
optimization of fermentation process
作者简介
桑秀梅，硕士研究生。E．mail：1571343401@qq．com
殷志敏，博士，教授，博士生导师。E—mail：yinzhimin@njnu．edu．cn，
