摘要
目的探讨严重生精障碍患者精子DNA碎片指数与血清Hcy水平的相关性研究。方法 2015年12月~2017年2月期间就诊的严重生精障碍男性患者56例,按WHO标准分为严重无、少精子症组25例和弱精子症组31例,对照组为无生育障碍的男性27例。对所有纳入研究者运用全自动精液分析仪进行精子参数分析,精子DNA碎片指数和血清Hcy水平的检测分别运用精子染色质扩散法和酶比色法。结果对照组人群中精子DNA碎片指数和Hcy水平的中位数分别为33%[95%CI(29.0%~34.4%)]和13.2μmol/L[95%CI(12.4μmol/L^14.2μmol/L)],而在严重生精障碍组中这两个指标分别为21%[95%CI(19.0%~24.0%)]和8.9μmol/L[95%CI(8.4μmol/L^9.4μmol/L)],这两个项目的检测结果对照组均高于严重生精障碍组,差异有统计学意义(t=6.793~7.543,P=0.000)。精子存活率正常对照组和严重生精障碍组分别为71%[95%CI(67.8%~75.1%)]和57%[95%CI(52.3%~58.0%)],差异有统计学意义(t=-8.475,P=0.000)。精子DNA碎片指数与血清Hcy水平、精子浓度呈正相关,Passing-Bablok回归方程分别为:Y=10.705+0.053X,Y=21.071+0.286X,而Hcy水平和精子浓度呈负相关趋势。结论 Hcy水平和精子DNA碎片指数的升高,可能是严重生精障碍男性的重要病因,但其具体机制有待进一步研究。
Objective To investigate the relationship between the homocysteine,sperm DNA fragmentation index and sperm counts of male with severe impaired spermatoqenesis. Methods From December 2015 to February 2017,56 male patients with severe impaired spermatoqenesis were enrolled in the study. The patients were divided into two groups according to the WHO criteria： severe oligozoospermia and azoospermia group （n = 25） and oligoasthenoteratozoospermia group （n = 31）, and the control group was a male with no reproductive impairment （n=27）. The sperm parameters were analyzed by using the computer automatic semen analyzer, sperm DNA fragmentation index and serum Hcy level were detected by sperm ehromatin diffusion method and enzyme colorimetric method. Results The median of Sperm DNA fragmentation index and ho- mocysteine level in control groups were 33% [95%CI（29.0%-34.4%）] and 13.2 μmol/L [95%CI（12.4 μmol/L-14.2 μmol/L）] ,and in severe spermatogenesis groups in these two indicators were 21% [95%CI（19.0%-24.0%）] and 8.9 mol/L [95% CI （8.4 μmol/L- 9.4μmol/L）], respectively. The results of these two items were higher than the control group, the difference was statistically significant （t= 6. 793 - 7. 543, P = 0. 000）. Sperm survival rate in normal control group and severe spermatogenesis group was 71% [95%CI（67.8%-75.1%）] and 57%[95%CI（52.3%-58.0%）],respectively,and the difference was statistically significant （t=-8. 475, P=0. 000）. Sperm DNA fragmentation index was positively correlated with serum Hcy level and sperm concentration, Passing-Bablok regression analysis was ： Y= 10. 705 ＋ 0. 053 X, Y= 21. 071＋0. 286X,and Hcy level was negatively correlated with sperm concentration. Conclusion The increase of Hcy level and sperm DNA fragmentation index may be an important cause of male with severe impaired spermatoqenesis,but the specific mechanism remains to be further studied.
出处
《现代检验医学杂志》
CAS
2017年第4期83-86,共4页
Journal of Modern Laboratory Medicine
基金
深圳市宝安区医疗卫生科研项目(2016CX300)
关键词
严重生精障碍患者
精子DNA碎片指数
同型半胱氨酸
patients with severe spermatogenic dysfunction
sperm DNA fragmentation index
homocysteine
作者简介
何海洪（1983-），男，在职硕士，主管技师，主要从事临床血液学和遗传学，E—mail：hehaihong@163．com。
通讯作者：周义文，男，教授，博士，主任技师，硕士生导师，E-mail：yiwenzhou21@aliyun．com。
