摘要
为了阐明哈萨克斯坦传统发酵乳制品中乳酸菌生物多样性,采用传统纯培养方法对采集的5份传统发酵乳制品中的乳酸菌进行分离纯化,运用16S rRNA基因序列分析方法和系统发育树研究进行属种鉴定。结果表明,从5份传统乳制品中分离鉴定了49株乳酸菌分属于4个属8个种或亚种,乳杆菌属(Lactobacillus)44株,片球菌属(Pediococcus)1株、肠球菌属(Enterococcus)3株,链球菌属(Streptococcus)1株。其中乳杆菌属、片球菌属、肠球菌属、链球菌属是哈萨克斯坦地区传统发酵乳制品中的主要乳酸菌,其中Pediococcus pentosaceus为优势菌种,占总分离株的22%。
The aim of this paper is to research the diversity of Lactic add bacteria in traditional fermented milk products from Kazakhstan. Lactic acid bacteria were isolated and purified by traditional pure culture form traditional fermented milk products samples, and then all isolates were identified by 16S rRNA gene sequence analysis. A total of 49 strains of Lactic acid bacteria were classified into 4 genera and 8 species or subspecies, namely Lactobacillus （44 strains）, Pediococcus （1 strains）, Enterococcus （3 strains）, Streptococcus （1 strain）. Pediococcus pentosaceus was regarded as the dominant species, occupied 22 % of the total isolates.
作者
刘红新
呼斯楞
刘文俊
孙天松
LIU Hongxin HU Sileng LIU Wenjun SUN Tiansong(Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018, China)
出处
《中国乳品工业》
CSCD
北大核心
2017年第7期9-12,共4页
China Dairy Industry
基金
国家科技支撑计划项目(2013BAD18B01)
国家自然科学基金课题(31471711)
作者简介
刘红新（1991-），女，硕士研究生，研究方向为微生物生理与发酵工艺。
