摘要
以印度南瓜"北观"幼苗的下胚轴、子叶、真叶、茎段为外植体,采用离体再生方法,研究了不同种类激素对愈伤组织发育及离体再生的影响,以期为建立高效的南瓜再生体系奠定基础。结果表明:以印度南瓜幼苗的下胚轴、子叶、真叶、茎段为外植体均可诱导出愈伤组织,其中子叶和茎段较易诱导出愈伤组织;愈伤组织在MS+1.0mg·L^(-1) 6-BA(或2.0mg·L^(-1) KT)+0.2mg·L^(-1) NAA培养基上进行继代培养效果较好,可获得质量较好的愈伤组织;在诱导愈伤组织再生培养中,通过不同激素配比处理外植体均未分化出不定芽,仅部分分化出不定根。
The hypocotyls,cotyledons,true leaves and stem segments of C.maxima‘Beiguan’inbred seedlings were used as explants,regenenration in vitro method was used to study the effects of different kinds of hormones on the development of callus in subculture,and to explore the regeneration of callus in vitro respectively for constructing high efficient regeneration system by callus way of pumpkin.The results showed that the callus could be induced from the hypocotyls,cotyledons,true leaves and stem segments of C.maxima‘Beiguan’inbred seedlings,and cotyledons and stem as explants were easier to induce callus than hypocotyls and true leaves.MS＋1.0 mg·L-1 6-BA（or2.0mg·L-1 KT）＋0.2mg·L-1 NAA medium was better in callus subculture by obtaining better quality of the callus.In the induction of callus regeneration culture,none the adventitious bud was inducted from all callus explants with different hormone combinations,only part of the callus differentiated into adventitious roots.
出处
《北方园艺》
CAS
北大核心
2017年第15期42-47,共6页
Northern Horticulture
基金
国家农业科技成果转化资金资助项目(2013GB2D000301)
河南科技学院2014年自然科学重大培育资助项目(ZD2014003)
作者简介
鲁晓晓（1991-），女，硕士研究生，研究方向为蔬菜种质资源与育种。E-mail：luxiaoxia0914@163．com．
责任作者：周俊国（1967-），男，博士，教授，现主要从事园艺植物育种与蔬菜栽培生理等研究工作。E-mail：jun-guol020@163．com．
