摘要
目的:探讨参元益气活血胶囊(参元丹)对急性心肌梗死(AMI)/抑郁大鼠血清5-羟色胺(5-HT)、肿瘤坏死因子-α(TNF-α)水平的影响,以研究益气逐瘀法对心梗合并抑郁神经体液的影响机制。方法:将30只Sprague-Dawley(SD)大鼠随机分为正常组、模型组、参元丹高剂量组、参元丹低剂量组、对照组,每组各6只,以冠脉结扎法及慢性轻度不可预知性刺激合并孤养法制作AMI/抑郁模型,分别予参元丹高剂量、参元丹低剂量、氟西汀或蒸馏水灌胃18 d。造模成功3 d后处死,测量各组大鼠血清5-HT、TNF-α水平。结果:血清5-HT浓度比较:与正常组比较,模型组大鼠血清5-HT浓度显著降低(P<0.05),与模型组比较,参元丹干预组大鼠血清5-HT浓度均显著升高(P<0.05)。参元丹干预组与对照组比较,血清5-HT浓度均无显著性统计学意义(P>0.05)。血清TNF-α浓度比较:与正常组比较,模型组大鼠TNF-α浓度无显著性统计学意义(P>0.05)。同模型组比较,对照组、参元丹干预组大鼠血清TNF-α浓度差异亦未呈现出统计学意义(P>0.05),但L-SYD血清TNF-α较各组明显升高。结论:参元丹能改善AMI/抑郁的血清5-HT水平下降的情况,但对血清TNF-α水平无显著影响。
Objective：To observe the effect of Shen Yuan Dan（SYD） on 5-HT in the serum of Sprague-Dawley （SD） rats with acute myocardial infarction（AMI）/depression,and study the mechanisms of the theory Enhancing-qi in Removing-stasis in treating AMI/depression.Methods：Thirty SD rats were allocated into normal group,model group,H-SYD（high dose SYD） group,L-SYD（Low dose SYD） group and control group randomly.The model was estabished by coronary artery ligation with chronic mild unpredictable stimulation and fed alone.Models were given high dose of SYD,low dose of SYD,fluoxetine and distilled water.All the models were put into death 3 days later.The levels of 5-HT and TNF-α in rats serum of different groups were measured.Results：1） Serum 5-HT concentration.Compared with the normal group,5-HT concentration in the model group significantly decreased （P〈0.05）;The serum concentration of 5-HT between the SYD group was significantly higher than that of the model group （P〈0.05）.The concentration of 5-HT in serum of SYD group and control group had no statistical difference （P〉0.05）.2） Serum TNF-α concentration.Compared with the normal group,the serum TNF-α concentration in the model group has no statistical difference （P〉0.05）,besides,TNF-α in serum of L-SYD group was higher than those of the normal group,control group and H-SYD group but without any significant difference（P〉0.05）.Conclusion：SYD can reduce the level of 5-HT in serum but failed to affect TNF-α level.
出处
《世界中医药》
CAS
2017年第2期251-253,共3页
World Chinese Medicine
基金
国家自然科学基金应急管理项目(编号:81541074)
作者简介
通信作者：刘红旭，男，博士研究生导师，研究方向：心血管病的中西医结合研究，E-mail：lxh_263．net
