摘要
目的探讨不同药物预处理对心肌梗死(心梗)、抑郁、心梗+抑郁大鼠血清、血小板、脑组织中的5-羟色胺(5-HT)水平的影响,以研究药物预处理对心梗和抑郁的保护作用。方法将80只4周龄Sprague-Dawley(SD)大鼠随机分为治疗组和对照组,每组各40只,分别予益气活血、改善微循环的中成药(主要成分三七、人参等)或生理盐水灌胃4周后,将治疗组和对照组再随机各分为4个亚组,每各亚组各10只,分别建立假手术、抑郁、心梗、心梗+抑郁四种动物模型,最终每组中建模成功的大鼠数量如下,对照组:假手术组亚组8只(80%),抑郁亚组9只(90%),心梗亚组8只(80%),心梗+抑郁亚组8只(80%);治疗组:假手术亚组9只(90%),抑郁亚组10只(100%),心梗亚组9只(90%),心梗+抑郁亚组8只(80%)。造模成功3 d后处死,测量各组大鼠血清、血小板及脑组织中5-HT水平。结果对照组的心梗、抑郁、心梗+抑郁亚组的血清5-HT水平、血小板5-HT水平均较假手术亚组下降(P<0.05);对照组的心梗亚组较假手术亚组脑组织5-HT水平下降,抑郁、心梗+抑郁亚组的5-HT水平,均较假手术亚组上升(P均>0.05)。治疗组的心梗、抑郁、心梗+抑郁亚组的血清、脑组织5-HT水平,均较假手术组显著上升,(P均<0.05);治疗组心梗、抑郁、心梗+抑郁亚组的血小板5-HT水平,均较假手术组下降(P均<0.05)。治疗组的假手术、心梗、抑郁及心梗+抑郁亚组的血清5-HT及血小板5-HT水平,均分别较对照组的相应的4个亚组高(P均<0.05)。治疗组的假手术、心梗、抑郁及心梗+抑郁亚组的脑组织5-HT水平,均分别较对照组的相应的4个亚组低,但除抑郁亚组外其余差异均有统计学意义(P均<0.05)。结论本研究的药物预处理能改善心梗、抑郁、心梗+抑郁等应激造成的血清、血小板5-HT水平下降的情况,但对脑组织5-HT水平无显著影响。
Objective To explore the effect of different pre-treatments on serum 5-HT, platelet 5-HT and brain 5-HT in the Sprague-Dawley rats of myocardial infarction（MI）, depression, and myocardial infarction coexist with depression（MI ＋ depression）, and study the effect of the drug in preventing MI and depression. Methods 80 SD rats were allocated into pretreatment group and control group randomly. After 4 weeks＇ pretreatment with Chinese patent medicine for benefiting qi and activing blood circulation and improving microcirculation system（include panax and ginseng etc.）, then both of the two groups were established four models of shame subgroup, depression subgroup, myocardial infarction subgroup, MI ＋ depression subgroup. The number of rats successfully modeled in control group was as follows： 8 rats in shame subgroup（80%）, 9 rats in depression subgroup（90%）, 8 rats in myocardial infarction subgroup（80%） and 8 rats in MI ＋ depression subgroup（80%）. The number of rats successfully modeled in pretreatment group was as follows： 9 rats in shame subgroup（90%）, 10 rats in depression subgroup（100%）, 9 rats in myocardial infarction subgroup（90%） and 8 rats in MI ＋ depression subgroup（80%）. All the rats were sacrificed 3 days later. 5-HT in serum, platelet, and brain of the rats in different groups were measured. Results In control group, 5-HT in serum and platelet decreased significantly in MI, depression and MI ＋ depression subgroup compared with shame subgroup（all P〈0.05）; 5-HT in brain of MI subgroup was lower than the shame subgroup, while the depression and MI ＋ depression were higher, but without any significance（all P〈0.05）. In pretreatment group, 5-HT in serum and brain increased significantly in MI, depression and MI ＋ depression subgroup compared with shame subgroup（all P〈0.05）; 5-HT in platelet of MI, depression and MI ＋ depression subgroup were lower than the shame subgroup（all P〈0.05）. In pretreatment group, 5-HT in serum and platelet of the four subgroups were significantly higher than in control group（all P〈0.05）; 5-HT in brain of the four subgroups were lower than the control group and there was significance（P〈0.05） except depression subgroup（P〈0.05）.Conclusions In this study, drugs could improve the decrease of 5-HT in serum and platelet due to myocardial infarction, depression and myocardial infarction co-exist with depression, but failed to influence 5-HT in brain.
出处
《中国循证心血管医学杂志》
2016年第12期1458-1462,共5页
Chinese Journal of Evidence-Based Cardiovascular Medicine
关键词
心肌梗死
抑郁
5-羟色胺
Myocardial infarction
Depression
5-HT
作者简介
通讯作者：刘梅颜,E-mail：china_lmy@hotmail.com
