摘要
目的研究胡柚皮总黄酮的大孔树脂纯化工艺。方法通过对8种不同型号大孔树脂进行静态吸附与解吸实验,优选合适的大孔树脂,并优化分离纯化条件。结果 HPD-300型大孔树脂对胡柚皮总黄酮有较好的吸附和洗脱效果,其最佳分离纯化条件如下:pH 4.0,3.83 mg·m L^(-1)上样液,上样量为5 m L·g^(-1),上样体积流量为2.0 BV·h^(-1),依次用2 BV的水洗脱,4 BV 70%乙醇洗脱,洗脱剂的流速为2 BV·h^(-1)。经HPD-300树脂处理后的总黄酮质量分数达76.21%,收率为93.94%。结论 HPD-300型大孔树脂用于富集胡柚皮总黄酮效果最佳,是一种理想的分离纯化介质。
OBJECTIVE To investigate the purification technology of total flavonoids from the peels of Citrus Changshan-huyou Y.B. Chang by macroporous resin. METHODS Eight macroporous resins were chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters. RESULTS HPD-300 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH 4.0, sample mass concentration of 3.83 mg·mL-1, the loading amount of 5 mL·g^-1, the loading flow rate of 2.0 BV·h^-1. The sample was eluted by 2 BV of water and 4 BV of 70% ethanol respectively with the eluting flow rate of 2.0 BV·h^-1. The content of total flavonoids increased to 76.21% after the purification, and the yield was 93.94%. CONCLUSION HPD-300 is an ideal resin with satisfactory enrichment for separating and purifying the total flavonoids from peels of Citrus Changshan-huyou Y.B. Chang.
出处
《中国现代应用药学》
CAS
CSCD
2017年第5期649-653,共5页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金(81573761)
浙江省自然科学基金(LY13H290011)
浙江省中医药科学研究基金(2015ZA074
2017ZB043)
杭州市医疗卫生科研项目(20150633B59)
关键词
胡柚皮
总黄酮
大孔树脂
纯化工艺
吸附动力学特性
peels of Citrus Changshan-huyou Y.B. Chang
total flavonoids
macroporous resin
purification technology
adsorption kinetics characteristics
作者简介
舒祝明，男，副主任药师Tel：17757506006E-mail：jim917@163．com
通信作者：蒋剑平，男，博士，副主任中药师Tel：（0571）86611022E—mail：jiangjp77@163．com
