摘要
目的以传统国标培养法作为参考比对方法,考察实时荧光核酸恒温扩增(simultaneous amplification and testing,SAT)食品检测试剂盒,在食品中检测食源性致病菌如志贺氏菌、大肠埃希氏菌O157∶H7/NM、阪崎肠杆菌的一致性。方法以食品中志贺氏菌、大肠埃希氏菌O157∶H7/NM和阪崎肠杆菌国标培养法为参考方法,以基因测序法作为确认法,考量SAT食品检测试剂盒方法的灵敏度、特异性、假阳性率、假阴性率、准确度,同时进行2种方法的显著差异检验。结果 3种SAT食品检测试剂盒灵敏度均为100%,假阴性为0;特异性:志贺氏菌100%,阪崎肠杆菌100%,大肠埃希氏菌O157∶H7/NM 98.4%。大肠埃希氏菌O157∶H7/NM假阳性率为1.2%。结论 SAT试剂盒检测方法具有灵敏度高、特异性强、准确度高的优点,无一例漏检。
Objective To evaluate the consistency of simultaneous amplification and testing（SAT） in detection of foodborne pathogenic microorganisms such as Shigella,Escherichia coli O157：H7/NM and Enterobacter sakazakii with the traditional national standard culture method as a reference alignment method.Methods The sensitivity,specificity,false positive rate,false negative rate and accuracy of 3 SAT kits for Shigella,Escherichia coli O157：H7/NM and Enterobacter sakazakii were investigated with the national standards method as reference and the method of molecular sequencing as validation.Meanwhile,the significant difference of positive rate was analyzed by the chi-square test.Results The sensitivities of 3 SAT kits were all 100%,and there were no false negative and misdetection rate.The specificities of Shigella,Escherichia coli O157：H7/NM and Enterobacter sakazakii were 100%,100%,and 98.4%,respectively,which all conformed to the requirements of the test.The false positive rate of Escherichia coli O157：H7/NM was 1.2%.Conclusion The SAT method has the advantages of high sensitivity,specificity,and accuracy,and has no missing detection.
出处
《食品安全质量检测学报》
CAS
2017年第3期1047-1052,共6页
Journal of Food Safety and Quality
关键词
实时荧光核酸恒温扩增
食品微生物检测
灵敏度
特异性
准确度
simultaneous amplification and testing
food microbiological detection
sensitivity
specificity
accuracy
作者简介
陈秀云，助理工程师，主要研究方向为微生物检测技术。E-mail：chenxiuyun@yijianyixue.com
通讯作者：焦红，研究员，主要研究方向为食品安全与营养学检测技术。E-mail：jhciq1228@163.com.焦红，研究员，主要研究方向为食品安全与营养检测技术。E-mail：jhciq1228@163.com
