摘要
目的:研究舒尼替尼对肾癌细胞体外生长、侵袭及Wnt/β-catenin信号通路的影响。方法:培养肾癌细胞株ACHN并用不同剂量舒尼替尼(1、2、4、8μmol/L)进行处理,以不含舒尼替尼的处理条件作为阴性对照。处理后24h时,测定细胞中凋亡基因、侵袭基因、Wnt/β-catenin信号通路的mRNA表达量。结果:处理后24h时,1、2、4、8μmol/L舒尼替尼组中NPRL2、Bax、caspase-3、caspase-9的mRNA表达量显著高于阴性对照组,MMP2、MMP9、Vimentin、N-cadherin、Wnt、β-catenin的mRNA表达量显著低于阴性对照组;舒尼替尼剂量越高,细胞中NPRL2、Bax、caspase-3、caspase-9的mRNA表达量越高,MMP2、MMP9、Vimentin、N-cadherin、Wnt、β-catenin的mRNA表达量越低。结论:舒尼替尼能够通过抑制肾癌细胞中Wnt/β-catenin信号通路来增加凋亡基因的表达、抑制侵袭基因的表达,进而抑制细胞的生长和侵袭。
Objective; To study the effect of sunitinib on renal cancer cell growth in vitro and invasion as well as Wnt/β- catenin signaling pathway. Methods： Renal cancer cell lines ACHN were cultured and processed with different doses of sunitinib （1 μmol/L, 2 μmol/L, 4 μmol/L and 8 μmol/L）, and sunitinib-free processing condition was used as negative control. Twenty-four hours after processing, the mRNA expression levels of apoptosis genes, invasion genes and Wnt/β-catenin signaling pathways in cells were detected. Results.. Twenty-four hours after treatment, NPRL2, Bax, caspase-3 and caspase-9 mRNA expression in 1 μmol/L, 2μmol/L, 4 /zmol/L and 8μmol/L sunitinib groups were significantly higher than those in negative control group while MMP2, MMPg, Vimentin, N-cadherin, Wnt and β-catenin mRNA expression were significantly lower than those in negative control group; the higher the dose of sunitinib, the higher the NPRL2, Bax, caspase-3 and caspase-9 mRNA expression while the lower the MMP2, MMPg, Vimentin, N-cadherin, Wnt and β-catenin mRNA expression in cells. Conclusions ： Sunitinib can inhibit the Wnt/β-catenin signaling pathway in renal cancer ceils to increase the expression of apoptosis genes, inhibit the expression of invasion genes and thereby inhibit the cell growth and invasion.
出处
《海南医学院学报》
CAS
2017年第8期1034-1036,1040,共4页
Journal of Hainan Medical University
基金
北京市科技计划课题(Z151100004015194)~~
作者简介
杨渝（1962-），男，重庆人，学士，副主任医师，电话：010—66848142、13910103589，Email：potato714@163．com。
