摘要
目的:探讨四制香附贴膏灸剂对缩宫素所致痛经模型大鼠血液流变性的影响,并初步探究其体外透皮特性。方法:SD大鼠随机分为空白组、模型组、四制香附贴膏灸剂组(贴膏灸剂组)及四制香附石油醚部位浸膏组(浸膏组)。通过灌胃己烯雌酚增敏,观察腹腔注射缩宫素后30 min内大鼠扭体发生的潜伏期及次数,测试各组大鼠的全血黏度和血浆黏度;采用改良的Franz扩散池,以小鼠离体皮肤为渗透屏障进行体外透皮试验,利用HPLC同时测定α-香附酮和香附烯酮的体外透皮渗透量,分别对四制香附贴膏灸剂组和不加发热辅助材料的四制香附贴膏剂组进行研究。结果:贴膏灸剂组和浸膏组均显著延长大鼠扭体反应发生的潜伏期,显著减少30 min内大鼠扭体发生次数,且贴膏灸剂组优于浸膏组;贴膏灸剂组和浸膏组均可显著降低痛经模型大鼠的全血黏度和血浆黏度,贴膏灸剂组更佳。四制香附贴膏灸剂中香附烯酮和α-香附酮24 h内的渗透速率分别为58.58,45.42μg·cm^(-2)·h^(-1),均高于不加发热辅助材料的贴膏剂组,透皮行为符合Higuchi方程。结论:四制香附贴膏灸剂可显著提高四制香附抗痛经有效成分的透皮释药性能,对大鼠痛经模型有良好的镇痛作用,可显著改善痛经大鼠的血液流变性。
Objective： To discuss the effect of thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma on hemorheology of dysmenorrhea rats caused by oxytocin and to investigate its in vitro transdermal absorption properties. Method： SD rats were randomly divided into 4 groups, such as the blank group, model group, thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma group and petroleum ether extract of Jianchangbang Cyperi Rhizoma group. The dysmenorrhea rats model were made by oxytocin and diethylstilbestrol, then were observed the times and incidence of wrings within 30 minutes. The blood viscosity and plasma viscosity of rats in all groups was tested by automatic blood rheometer. The percutaneous permeation of α-cyperone and cyperotundone in the thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma was studied by in vitro transdermal delivery experiment with Franz diffusion cells and their contents were determined by HPLC. Result： Thermal moxibustion emplastrum group and petroleum ether extract group could significantly reduce the times and incidence of wrings within 30 minutes, and the thermal moxibustion emplastrum group was better than the petroleum ether-extract group. Compared with the blank group, the model group significantly increased the whole blood viscosity and plasma viscosity, but the thermal moxibustion emplastrum group and the petroleum ether-extract group could significantly reduce them of model rats, also the thermal moxibustion emplastrum was better. The permeation rates of α-cyperone and cyperotundone from the thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma were 45.42, 58.58 μg·cm-2·h-1, and their transdermal behavior conformed to Higuchi equation. Conclusion： Thermal moxibustion emplastrum with Jianchangbang Cyperi Rhizoma can significantly improve the drug release properties of active ingredients with auxiliary heating materials, it can effectively relieve oxytocininduced pain in rats and improve hemorheology of dysmenorrhea rats.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第11期29-33,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省科技厅项目(20151BAB215035
20161BBG70112)
江西省高校落地计划产学研合作项目(KJLD14057)
江西省卫计委中医药科研计划重点课题(2016Z05)
江西中医药大学研究生创新基金项目(JZYC16S04)
关键词
四制香附
贴膏灸剂
血液流变学
累积透过量
香附烯酮
Α-香附酮
镇痛作用
Cyperi Rhizoma with Jianchangbang
thermal moxibnstion emplastrum
hemorheology
cumulative penetration
cyperotundone
α-cyperone
analgesic effect
