摘要
筛选自贵州侗族酸肉中产胞外多糖的乳酸菌SR2-2,利用单因素结合正交试验对其产胞外多糖的最佳发酵条件及其提取工艺进行研究。结果表明,最佳发酵工艺为蔗糖2%、胰蛋白胨∶蛋白胨=2∶1、发酵温度31℃、发酵时间24 h,在此条件下胞外多糖产量为(565.52±6.81)mg/L,较优化前提高了58.32%;胞外多糖最佳提取工艺为:三氯乙酸终含量为10%、加入24 m L无水乙醇沉淀18 h,在此条件下多糖提取量为(790.22±13.00)mg/L,较优化前提高了53.31%。
The exopolysaccharide-producing lactic acid bacteria SR2-2 was isolated from sour meat of the Dong Minority in Guizhou. The optimum exopolysaccharide-producing fermentation conditions and extraction process of exopolysaccharide were researched by single factor and orthogonal experiments. The results showed that optimum fermentation conditions were sucrose 2%, tryptone：peptone 2：1, fermentation temperature 31 ℃, fermentation time 24 h. Under the conditions, exopolysaccharide yield was （565.52±6.81） mg/L, which was 58.32% higher than that of before optimization. The optimum extraction process of exopolysaccharide was trichloroacetic acid （TCA） final concentration 10%, absolute ethyl alcohol 24 ml, alcohol precipitation time 18 h. Under the conditions, polysaccharide extraction yield was （790.22±13.00） mg/L, which was 53.31% higher than that of before optimization.
出处
《中国酿造》
CAS
北大核心
2017年第5期118-122,共5页
China Brewing
基金
国家自然科学基金项目(31260379)
关键词
乳酸菌
胞外多糖
发酵条件
提取工艺
优化
lactic acid bacteria
exopolysaccharide
fermentation conditions
extraction process
optimization
作者简介
廖乾伟（1991-），女，硕士研究生，研究方向为食品微生物与畜产品加工。
通讯作者：胡萍（1970-），女，教授，博士，研究方向为畜产食品与食品微生物。
