摘要
采用HPLC法分析了武夷水仙茶、建阳水仙茶、建瓯水仙茶和永春水仙茶等170个茶样的儿茶素组分以及生物碱组分,运用PCA、FDA、KNN以及PLS-DA方法进行判别分析.结果表明:武夷水仙茶、建阳水仙茶、建瓯水仙茶的生物碱总量以及酯型儿茶素中没食子儿茶素没食子酸酯(GCG)显著高于永春水仙茶,其他酯型儿茶素、简单儿茶素中的表儿茶素(EC)以及表没食子儿茶素(EGC)低于永春水仙茶;在基于儿茶素及生物碱组分对不同产地的判别区分中,FDA判别模型效果最好,武夷水仙茶、建阳水仙茶、建瓯水仙茶、永春水仙茶的正确判别率分别为96.0%、100%、100%、100%,总样品的判别准确率达到98.8%,准确率较高.说明采用FDA建立的儿茶素组分以及生物碱组分判别模型具有较好的实际预测效果和应用价值,可实现对福建水仙茶产地的高准确率识别.
In order to distinguish different places of origin of Shuixian tea, catechins and alkaloid contents of 170 tea samples, including Wuyi Shuixian, Jianyang Shuixian, Jianou Shuixian and Yongchun Shuixian, were analyzed by HPLC. Simultaneously, discriminant analysis was performed using PCA, FDA, KNN and PLS-DA methods. The results showed that total alkaloids and ester-type catechin in the gallocatechin gallate（GCG）of Wuyi Shuixian, Jianyang Shuixianand Jianou Shuixian catechins were significantly higher than those in Yongchun Shuixian, while other ester catechins, simple tea catechin epicatechin（EC）, and table epigallocatechin（EGC）were lower than those in Yongchun Shuixian. FDA discriminant model proved to be the best model for analysis based on catechins and alkaloid contents, with correct discrimination rates of Wuyi Shuixian, Jianyang Shuixian, Jianou Shuixian and Yongchun Shuixian being 96.0%, 100%, 100% and 100% respectively and accuracy being 98.8%. To summarize, FDA discriminative model based on catechins and alkaloid contents is highly applicable to identify place of origin with high accuracy.
作者
邱晓红
张丹丹
陈静
王鹏杰
杨国一
王丽
郑德勇
叶乃兴
QIU Xiaohong ZHANG Dandan CHEN Jing WANG Pengjie YANG Guoyi WANG Li ZHENG Deyong YE Naixing(College of Horticulture,Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou,Fujian 350002,China College of Tea and Food Science,Wuyi University, Wuyishan,Fujian 354300,China)
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2017年第3期260-264,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金资助项目(31270735)
福建省"2011协同创新中心"专项资助项目(闽教科[2015]75号)
福建茶产业农技推广服务试点建设资助项目(KNJ-151001)
关键词
福建水仙
乌龙茶
产地
儿茶素
生物碱
模式识别
Camellia sinensis cv. Fujan Shuixian
Oolong tea
place of origin
catechin component
alkaloids
pattern recognition
作者简介
邱晓红（1993-）,女,硕士研究生.研究方向：茶叶品质化学与生物技术.Email：1289203149@qq.com.
通讯作者叶乃兴（1963-）,男,教授,硕士.研究方向：茶树栽培育种与品质调控.Email：ynxtea@126.com.
