摘要
目的采用基于16S核糖体DNA(r DNA)的高通量测序技术,分析10例慢性牙周炎患者接受龈下刮治和根面平整术(SRP)治疗前后龈下菌斑多样性及相对丰度的变化,探讨应用微生物群落的构成变化作为牙周炎诊断及预后评估指标的可行性。方法选择2014年3—9月在首都医科大学附属北京口腔医院牙周科就诊的10例慢性牙周炎患者作为研究对象,在SRP治疗前及治疗后3个月分别在研究对象的同一位点采集龈下菌斑样本,提取样本基因组DNA,采用Illumina Miseq平台测序,分析各组样本从门到种各水平的菌群分布及相对丰度。结果在门水平上,共检测到16个菌门,有8个门的细菌在牙周龈下菌斑菌群结构中占主要地位(99%);在属水平上,共检测到128个不同菌属,SRP治疗后3个月坦纳菌属(Tannerella)的相对丰度较治疗前明显降低(P<0.05),纤毛菌属(Leptotrichia)和链球菌属(Streptococcus)的相对丰度较治疗前明显上升(P<0.05);在种水平上,6种牙周可疑致病菌被检出,SRP治疗后3个月福赛坦纳菌(Tannerella forsythia)和中间普氏菌(Prevotella intermedia)的相对丰度较治疗前明显减少(P<0.05)。结论慢性牙周炎患者龈下菌群具多样性,SRP治疗前后,牙周可疑致病菌的相对丰度降低,而有益菌的相对丰度升高,SRP治疗可以明显改变龈下菌群构成。
Objective This study aims to observe the changes in the microbiological community of a subgingival plaque in chronic periodondotis after subgingival scaling and root planing（SRP）. The observation was performed via high- throughput sequencing. The feasibility of this approach in diagnosis and prognosis in chronic periodontitis was then explored by microflora transition. Methods Ten patients with chronic periodontitis under treatment in the Periodontics Department of Beijing Stomatological Hospital were selected in this study. From each patient, we obtained genomic DNA from the subgingival plaque before SRP and then three months after SRP in the same sampling site. High-throughput sequencing analysis was then performed to investigate the diversity and relative abundance of the subgingival plaque microflora from phyla to species.Results In the phylum level, 16 phyla were detected, of which eight constitute 99% of the sequences in the subgingival plaque samples from each time point. In the genus level, 128 different genera were detected in all time points. The results showed that the relative abundance of Tannerella decreased three months after SRP（P〈0.05）, whereas those of Leptotrichia and Streptococcus increased three months after SRP（P〈0.05）. In the species level, 6 putative pathogens were detected. The relative abundance of Tannerella forsythia and Prevotella intermedia decreased three months after SRP（P〈0.05）. Conclusion The microflora of a subgingival plaque in chronic periodondotis is diverse, and the relative abundance of pathogens decreased after SRP, whereas the probiotics increased. Thus, SRP can change the microbiological community.
出处
《国际口腔医学杂志》
CAS
CSCD
北大核心
2017年第3期294-300,共7页
International Journal of Stomatology
基金
首都临床特色应用研究(Z141107006614005)
北京口腔医院学科建设基金(13-09-17)~~
关键词
慢性牙周炎
龈下刮治和根面平整术
高通量测序
chronic periodontitis
subgingival scaling and root planing
high-throughput sequencing
作者简介
赵红，副主任技师，硕士，Email：harrietzha01021@126．com
[通信作者]苏建荣，主任医师，博士，Email：sujr9@sina．com
