摘要
以‘秦冠’、‘富士’、‘金冠’苹果果实为材料,通过对损伤接种灰葡萄孢菌(Botrytis cinerea Pers)后果肉组织酚类代谢主要产物和相关酶活性变化的分析测定,揭示苹果采后酚类物质代谢与灰霉病抗性的关系,为苹果灰霉病抗性鉴定和筛选抗灰霉病苹果资源提供理论指导。结果表明:(1)接种灰葡萄孢菌后,3个苹果品种的果实灰霉病发病率和病斑直径大小均为‘秦冠’<‘富士’<‘金冠’,而且3个品种间的发病率和病斑直径均差异显著,各品种对灰霉病的抗性由强到弱依次为‘秦冠’>‘富士’>‘金冠’。(2)抗病品种‘秦冠’果肉组织中类黄酮、木质素含量及苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)活性均显著高于感病品种‘富士’和‘金冠’,但总酚含量为‘秦冠’<‘金冠’<‘富士’,且3品种间总酚含量差异显著。研究表明,抗病苹果品种通过调节果肉内酚类物质代谢,增强次生代谢能力,其中类黄酮和木质素含量的增加强化了果实的抗性反应,进而提高对灰霉病的抗性,但总酚含量与植物抗病性关系不大。
Using‘Qinguan＇、‘Fuji＇、‘Golden delicious＇ as apple materials to analysis the changes of phenolic content and relevant enzyme activities of pulp by inoculation with Botrytis cinerea Pers.The results provide a physiological theoretical basis for apple gray mold resistance.The results indicated that：（1）the size of disease incidence and lesion diameter in three apple varieties after the inoculation were ‘Qinguan＇〈‘Fuji＇ 〈‘Golden delicious＇（P〈0.05）,so all varieties of grey mold disease resistance were‘Qinguan＇〉‘Fuji＇ 〉‘Golden delicious＇.（2）The phenylalanine ammonialyase（PAL）,peroxidase（POD）,poly phenol oxidase（PPO）activites in the pulp of resistant variety were higher than that in susceptible variety,and the contents of flavonoids and lignin were also higher significantly（P〈0.05）.Besides,the content of total phenolic compounds were‘Qinguan＇ 〈‘Golden delicious＇ 〈‘Fuji＇.In conclusion,resistant variety improved secondary metabolism by regulating phenolic metabolism to improve disease resistance in grey mold.The increasing content of flavonoids and lignin strengthened grey mold disease resistance of apple,but the total phenols content has little relation with the disease resistance.
出处
《西北植物学报》
CAS
CSCD
北大核心
2017年第3期510-516,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家现代苹果产业技术体系建设专项(nycylx-08-05-02)
关键词
苹果
品种
灰霉病
酚类代谢
抗性
apple
varieties
gray mold
phenolic metabolism
resistance
作者简介
唐永萍（1991-）,女,硕士,主要从事园艺产品采后生理及藏保鲜研究.E-mail：1540031309@qq.com
通信作者：周会玲,博士,副教授,主要从事园艺产品采后处理及藏保鲜研究.E-mail：zhouhuiling@nwsuf.edu.cn
