摘要
为了明确β-1,3-葡聚糖酶和几丁质酶与大豆抗疫霉根腐病的关系,测定了不同抗性大豆品种接种大豆疫霉菌后β-1,3-葡聚糖酶和几丁质酶活性的变化情况和2种酶对大豆疫霉菌的抑制作用。结果表明,大豆疫霉菌能诱导大豆β-1,3-葡聚糖酶和几丁质酶的活性增强,但2种酶在积累速度和幅度上,抗病品种和感病品种有显著的差异。与感病品种"857-1"相比,抗病品种"垦农4号"2种酶活性不仅升高的速度快、幅度大,且高活性维持的时间长。β-1,3-葡聚糖酶和几丁质酶混合液对大豆疫霉菌的菌丝生长、孢子囊形成和孢子萌发的抑制作用明显,其次是β-1,3-葡聚糖酶,而几丁质酶对大豆疫霉菌的抑制作用不明显。表明大豆对大豆疫霉根腐病的抗性与β-1,3-葡聚糖酶和几丁质酶的活性呈正相关关系。β-1,3-葡聚糖酶和几丁质酶混合对大豆疫霉菌的抑制具有协同增效作用。
To realize the correlation between activities of β-1,3-glucanase and chitinase induced by Phytophthora sojae and resistance of soybean to phytophthora root rot,the activities of the two enzymes after inoculation and their inhibition to P.sojae in soybean were studied.The results showed that the activities in soybean were increased by infection of P.sojae,but the increase in activities was faster and higher,and lasted longer in a resistant variety than that in susceptible varieties.β-1,3-glucanase and the mi...
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第6期600-607,共8页
Acta Phytopathologica Sinica
基金
植保资源与病虫害治理教育部重点实验室(西北农林科技大学)2006年访问学者基金
黑龙江省农垦总局科技项目(HNKX-IV-02-04-01)
