摘要
目的研究人参皂苷Rg_1对H_2O_2诱导的HT22细胞凋亡及胞内Ca^(2+)浓度变化的影响。方法以0、6.25、12.5、25、50、100μg/L人参皂苷Rg_1预处理细胞24 h,采用Ca^(2+)荧光染料探针Fluo-3/AM负载细胞1 h后,50 mmol/L H_2O_2刺激细胞,多功能酶标仪测定荧光强度,激光共聚焦显微镜实时监测[Ca^(2+)]i变化,Hoechst 33258染色检测细胞凋亡。结果 H_2O_2可诱导细胞内Ca^(2+)浓度增加(P<0.01),并增加细胞凋亡率(P<0.01)。不同浓度人参皂苷Rg_1可呈剂量依赖性的抑制H_2O_2诱导的HT22[Ca^(2+)]i增加(P<0.01),抑制细胞凋亡(P<0.01),以50μg/L的作用为最强(P<0.01)。结论人参皂苷Rg_1可通过降低H_2O_2诱导的HT22细胞内Ca^(2+)水平的升高,抑制氧化应激引起的细胞凋亡。
Objective To investigate the effect of ginsenoside Rg_1 on H_2O_2induced apoptosis and intracellular Ca^2＋concentration in HT22 cells. Methods Ginsenoside Rg_1 with 0, 6.25, 12.5, 25, 50 and 100 μg/L were used to pre-processing HT22 cells for 24 h, separately. Ca^2＋fluorescent probe Fluo-3/AM was used to load cells for 1h. Then using 50 mmol/L H_2O_2 stimulate cells. The fluorescence intensity was measured for microplate reader, [Ca2 ＋]i changes were monitored by scanning confocal microscopy. Apoptosis were tested by Hoechst 33258 staining. Results The Ca^2＋concentration（P〈0.01） and apoptosis（P〈0.01） induced by H_2O_2 increased significantly. Different concentrations of ginsenoside Rg_1 showed dose dependent inhibition in increasing the HT22 [Ca^2＋]i（P〈0.01） and apoptosis（P〈0.01） induced by H_2O_2, and the concentation of 50 μg/L shows strongest effect（P〈0.01）. Conclusion The ginsenoside Rg_1 could inhibit apoptosis of oxidative apoptosis by reducing the cellular Ca^2＋level induced by H_2O_2.
出处
《湖南中医药大学学报》
CAS
2017年第3期236-239,共4页
Journal of Hunan University of Chinese Medicine
基金
国家自然科学基金项目(81473581)
湖南省教育厅项目(14C0859)
中西医结合防治心脑疾病的相关基础研究湖南省高校科技创新团队项目(2015CXTD)
中西医结合基础湖南省重点学科开放基金项目
作者简介
刘晓丹,女,硕士,实验师,研究方向：心脑疾病中西医结合防治研究.
〔通讯作者〕邓常清,男,博士,教授,博士研究生导师,E-mail：dchangq@@sohu.com.
