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纤维素酶酶解工艺对香芹制汁效果的研究 被引量:3

Research on the optimization of the juice yield of Petroselinum crispum by technical enzymolysis of enzymatic hydrolysis
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摘要 四季香芹是伞形科欧芹属的一种高营养保健蔬菜,其营养丰富,香气浓郁,保健价值突出,是进行蔬菜汁加工的优质原料,但四季香芹出汁率不高是制约蔬菜汁加工的主要技术瓶颈之一。通过单因素试验分别研究纤维素酶添加量、酶解时间、酶解温度对香芹出汁率的影响,并通过Box-Behnken试验设计进行响应面分析试验优化,建立回归方程预测模型。研究表明纤维素酶在香芹制汁最佳应用条件为纤维素酶(15 000 U/g)添加量0.06%、酶解时间1.56 h、酶解温度40.14℃,香芹出汁率预测值为78.38%,与实际出汁率(78.72%±0.25%)符合度高。 Four-season celery( Petroselinum crispum) is one of high value nutrient and healthy vegetables,with rich nutrition,strong perfume and health care value and is high quality raw material for vegetable juice processing. but low yield of celery juice was one of the main technical bottlenecks restricting the vegetable juice processing. The effects of cellulase adding quantity,enzyme hydrolysis time and enzyme hydrolysis temperature on celery juice yield were studied separately,and response surface analysis was optimized by BoxBehnken design,and interaction of single factor on celery juice yield was analyzed. The regression equation prediction model was established in the paper. The optimum conditions for the application of cellulase in celery juice were as follows: cellulase addition quantity 0. 06%,enzymatic hydrolysis time 1. 56 h,enzymatic hydrolysis temperature 40. 14 ℃,and the yield of celery juice was 78. 38%. The actual yield of celery juice( 78. 72% ± 0. 25%) was better fitting of the predictive value.
出处 《上海海洋大学学报》 CAS CSCD 北大核心 2017年第2期307-313,共7页 Journal of Shanghai Ocean University
基金 上海市农产品保鲜加工服务平台项目(14DZ2293900) 上海市科技兴农推广项目(沪农科推字〔2015〕第5-1号)
关键词 四季香芹 出汁率 纤维素酶 响应面分析 four-season celery(Petroselinum crispum) juice yield cellulase response surface analysis
作者简介 乔勇进(1967-),男,研究员,博士后,研究方向为农产品保鲜加工。E-mail:yjqiao2002@126.com 通信作者:王新其,E-mail:wxqsaas@163.com
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