摘要
目的建立补肾强身片(淫羊藿、菟丝子、金樱子等)UPLC指纹图谱。方法该药物50%乙醇提取液的分析采用Agilent SB-C_(18)色谱柱(100 mm×4.6 mm,2.7μm);以乙腈-0.1%磷酸为流动相,梯度洗脱;体积流量0.5 mL/min;检测波长215 nm;柱温40℃。再通过聚类分析、主成分分析、正交偏最小二乘判别分析对指纹图谱进行评价。结果 12批样品的UPLC指纹图谱中有20个共有峰,并鉴定出9个(原儿茶酸、红景天苷、绿原酸、金丝桃苷、特女贞苷、朝藿定C、淫羊藿苷、山柰素、宝霍苷Ⅰ),相似度0.843~0.970。12批样品可分成3类,并找到4个差异标志物,包括特女贞苷和绿原酸。结论该方法简便可靠,可用于补肾强身片的质量控制。
AIM To establish the UPLC fingerprints of Bushen Qiangshen Tablets（Epimedii Folium,Cuscutae Semen,Rosae Laevigatae Fructus,etc.）.METHODS The analysis of 50% ethanol extract of this drug was performed on a 40 ℃ thermostatic Agilent SB-C18 column（100 mm×4.6 mm,2.7 μm）,with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelength was set at 215 nm.Then the fringerprints were evaluated by cluster analysis,principal components analysis and orthogonal partial least squares discriminant analysis.RESULTS There were twenty common peaks in the UPLC fingerprints of twelve batches of samples,nine of which（protocatechuic acid,salidroside,chlorogenic acid,hyperin,specnuezhenide,epimedin C,icariin,kaempferide and baohuoside Ⅰ） were identified,with the similarities of 0.843-0.970.Twelve batches of samples could be divided into three types,and four differential markers,including specnuezhenide and chlorogenic acid,were found out.CONCLUSION This simple and reliable method can be used for the quality control of Bushen Qiangshen Tablets.
作者
林秀莲
宋粉云
李华
黄寿辉
LIN Xiu-lian SONG Fen-yun LI Hua HUANG Shou-hui(School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China Guangdong Provincial Institute for Drug Control, Guangzhou 510180, China)
出处
《中成药》
CAS
CSCD
北大核心
2017年第3期551-555,共5页
Chinese Traditional Patent Medicine
基金
上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001)
关键词
补肾强身片
UPLC指纹图谱
聚类分析
主成分分析
正交偏最小二乘判别分析
Bushen Qiangshen Tablets
UPLC fingerprints
cluster analysis
principal components analysis
orthogonal partial least squares discriminant analysis
作者简介
林秀莲（1989-），女，硕士生，从事现代分析方法在中药质量控制中的应用研究。Tel：13418015391，E-mail：xiulianlin@163．com
通信作者：宋粉云（1965-），女，硕士，教授，从事现代分析方法在中药质量控制中的应用研究。Tel：（020）39352136，E-mail：songfenyun2011@163．com
