摘要
目的建立大鼠肝细胞癌(HCC)模型,观察青藤碱对肝细胞癌大鼠肝移植术后免疫应答的影响。方法将60只雄性Lewis大鼠用二乙基亚硝胺灌胃14周的方法建立大鼠肝细胞癌模型,成模后将用DA大鼠作为供体进行肝脏移植,术后随机分为模型组、青藤碱组。青藤碱组用青藤碱[2.0 mg/(kg·d)]灌胃,模型组灌等体积0.9%氯化钠注射液。采用ELISA法检测第1、2、3、4周肝脏组织中白介素-2(IL-2)、白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)及趋化因子-10(IP-10);采用流式细胞仪检测静脉血T细胞(Treg)、CD4^+T、CD8^+T等占T淋巴细胞的百分比。结果青藤碱组在第3周和第4周时,肝脏组织中IL-2、IL-6,静脉血Treg%、CD4^+T%明显降低,第4周时TNF-α、TGF-β1和IP-10明显降低,与模型组比较(P<0.05),且以上各指标在4周时的检测值与同组1、2、3周时比较,差异有统计意义(P<0.05)。结论青藤碱可抑制肝细胞癌大鼠肝移植术后细胞免疫。
Objective： To establish the rat model with hepatocellular carcinoma（HCC）,and to observe the effect of sinomenine on immune response in rats after liver transplantation for hepatocellular carcinoma. Methods： 60 male Lewis rats were made into the rat model with hepatocellular carcinoma with two diethylnitrosamine gastric irrigation method for 14 weeks. After modeling,DA rats after the operation of liver transplantation were randomly divided into the model group and sinomenine group. Sinomenine group received sinomenine gavage [2.0 mg/（kg·d）],while the model group were given normal saline. ELISA was used to detect interleukin-2（IL-2）,IL-6,IL-10,tumor necrosis factor（TNF-α）,transforming growth factor beta 1（TGF- beta 1） and chemokine IP-10 in the liver tissue in 1st,2nd, 3rd, and 4thweek;flow cytometry was used to detect the percentage of T blood cells（Treg）,CD4~＋T,CD8~＋T in T lymphocytes. Results： In third week and the fourth week,IL-2,IL-6,Treg% and CD4~＋T% in venous blood of liver tissue decreased significantly in sinomenine group. In the fourth week,TNF-a,TGF-β1 and IP-10 decreased significantly,compared with the model group（P〈0.05）. And there was significant difference in the detection values of indicators above in 4th weeks in the same group,compared with those in 1st,2nd and 3rd week（P〈0.05）. Conclusion： Sinomenine can inhibit cellular immunity in rats with hepatocellular carcinoma after liver transplantation.
出处
《中国中医急症》
2017年第2期222-225,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
湖北省教育指导项目(B2016125)
关键词
大鼠
青藤碱
肝细胞癌
肝脏移植
细胞免疫
免疫应答
Rat
Sinomenine
Hepatocellular carcinoma
Liver transplantation
Cellular immunity
Immune response
作者简介
通信作者（电子邮箱：qingqxjqxj@163．com）
