摘要
目的:探讨甲状腺激素(T3)对大鼠脑缺血再灌注损伤后Cyt C和AIF表达的影响及其机制。方法:雄性SD大鼠随机分为假手术组(sham1)、假手术组+T3(sham2)、缺血再灌注组(IR)、缺血再灌注组+T3(T3)4组。线栓法建立MCAO模型,缺血后1 h及再灌注6 h腹腔注射甲状腺激素10μg/100 g,生理盐水为安慰剂。缺血2 h,再灌注24 h后进行神经功能缺损评分;TTC染色测定脑梗死体积百分比;HE染色观察形态学变化;免疫组化技术和实时荧光定量PCR方法检测Cyt C及AIF阳性表达和m RNA水平。结果:与sham1、sham2相比,IR组Cyt C及AIF阳性表达及m RNA水平显著升高(P<0.01),而T3组较IR组显著下降(P<0.01),神经功能、梗死体积明显改善。结论:甲状腺激素对大鼠脑缺血再灌注损伤有一定保护作用,其机制可能与抑制促凋亡因子CytC及AIF表达有关。
Objective To investigate the effect of thyroid hormone（T3）on the expressions of cytochrome c （CytC） and apoptosis-inducing factor（AIF） after cerebral ischemia reperfusion injury in rats and its mechanism. Methods SD male rats were randomly divided into four groups： sham operation group（shaml）, sham operation group ＋ T3（sham2）, ischemia-reperfusion group （IR）, and thyroid hormone treatment group（T3）. A rat model of cerebral ischemia-reperfusion injury was established by right middle cerebral artery occlusion for 2 h, followed by reperfusion for 24 h. Thyroid hormones （10 μg/100 g） or normal saline were given at 1 h after onset of ischemia and 6 h after reperfusionby intraperitoneal injection. Neurological deficit scores were evaluated 24 h after reperfusion. Cerebral infarction volume was evaluated by TTC staining. Histological changes was observed by HE staining. Expressions and mRNA levels of CytC and AIF in ischemic brain tissue were evaluated by immunohistochemistry and real-time fluorescent quantitative RT-PCR. Results Ascompared with those in sham operation groups, the expressions and mRNA levels of CytC and AIFincreased significantlyin IR groups. As compared with those in IR groups, the indexes were remarkably decreasedin T3 groups （P 〈 0.01）. Nerve function was markedly improved andinfarction area narrowed. Conclusions Thyroid hormone plays a certain role in protection of cerebral ischemia- reperfusion injury in rats, whose mechanism may be associated with inhibition of the expression of apoptosis factors- CytC and AIF.
出处
《实用医学杂志》
CAS
北大核心
2017年第1期67-71,共5页
The Journal of Practical Medicine
基金
河南省医学科技攻关计划项目基金资助项目(编号:16A310021)
作者简介
通迅作者：刘恒方E-mail：Liuhf1965@163．com
