摘要
目的观察甲状腺激素T3预处理对小鼠缺血-再灌注(IR)肾脏白介素-10(IL-10)、内源性白介素-1受体阻滞剂(IL-1Ra)表达的调控及其对中性粒细胞浸润的影响,探讨T3对IR肾脏的保护作用及可能的机制。方法120只雄性C57BL/6小鼠随机分为4组(n=30):正常对照组(假手术)、单纯IR组(仅行肾脏IR)、T3+IR组(肾脏IR前48 h行T3预处理)、NaOH+IR组(肾脏IR前48 h注射等量0.1 mol/L NaOH),建立肾脏IR模型。各组于再灌注后24 h取血标本进行肾功能检测(肌酐、尿素氮);取肾脏标本采用PAS染色评估肾脏组织形态学改变,髓过氧化物酶(MPO)染色评估中性粒细胞浸润情况;real-time PCR检测肾脏再灌注后1、3、6、12、24、48 h的IL-10、IL-1Ra mRNA的表达。结果再灌注后24 h T3+IR组血肌酐、尿素氮水平较单纯IR组降低(P<0.05)、肾脏损伤程度轻(P<0.05);MPO染色示T3+IR组中性粒细胞浸润较单纯IR组明显减少(P<0.05)。再灌注后12 h,T3+IR组IL-10、IL-1Ra mRNA表达高于单纯IR组,并持续至再灌注后48h,差异有统计学意义(P<0.05)。单纯IR组和NaOH+IR组在上述各项指标的变化上无明显差异。结论 T3能在肾脏再灌注的晚期阶段发挥作用,促进IL-10、IL-1Ra的表达,减轻中性粒细胞浸润所产生的炎症反应,改善肾功能。
Objective To investigate the influence of thyroid hormone T3 preconditioning on interleukin-10（IL-10） and interleukin-1 receptor antagonist（IL-1Ra） modulation and neutrophil infiltration after renal ishchemia/reperfusion（IR） in mice,so as to study the protective effect of T3 on IR kidney.Methods Totally 120 male C57BL/6 mice were randomly divided into four groups（n=30）,namely,control group（sham operation）,IR group（only received renal IR）,T3＋IR group（T3 preconditioning for 48 h before renal IR）,and NaOH＋IR group（received equivalent 0.1 mol/L NaOH soltuion 48 h before renal IR）.The serum creatinine and blood urea nitrogen（BUN） were determined 24 h after reperfusion in each group;renal histological damages were scored using PAS staining;the levels of neutrophil infiltration were evaluated by MPO staining,and IL-10,IL-1Ra mRNA expression was examined by real-time PCR at 1,3,6,12,24,and 48 h after reperfusion.Results The serum creatinine and BUN levels of T3＋IR group were significantly lower than those of IR group 24 h after reperfusion（P〈0.05）,which was accompanied by lower histological score and significantly less neutrophil infiltration（P〈0.05）.Real-time PCR results showed that IL-10 and IL-1Ra mRNA expression in T3＋IR group was significantly higher than that in the IR group（P〈0.05） 12 h after reperfusion,which lasted for 48 h after reperfusion.The above parameters were similar between IR group and NaOH＋IR group.Conclusion Thyroid hormone T3 preconditioning can alleviate renal IR injury,partly by increasing expression of IL-10 and IL-1Ra and subsequently reducing neutrophil infiltration at the late phase of renal IR.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2012年第4期364-367,共4页
Academic Journal of Second Military Medical University
基金
上海市科委基金(09410706300)~~
作者简介
王盼梁，硕士生．E—mail：panliang133000@126．com
通信作者（corresponding author）．Tel：02168383755，Email：zhangjianjun@medmail．com．cn
