摘要
目的探讨多种套装制备的富血小板血浆(PRP)中的细胞和细胞因子成分,为临床医师和科研人员合理选择PRP制备套装提供依据。方法 2015年8月至2016年6月,盐城市第一人民医院烧伤整形科共招募20名健康志愿者捐献新鲜血液。将每份新鲜血液分为4小份,分别使用Arthrex、Regen、威高和Harvest四种套装制备PRP。使用血常规检测分析全血和PRP中的血小板和白细胞浓度,并以PRP中的血细胞浓度除以全血中的血细胞浓度计算PRP的血小板和白细胞富集度,以PRP中血小板总量除以全血中的血小板总量计算PRP的血小板和白细胞回收率。使用免疫酶联反应法检测激活2 h后的PRP中血小板源性生长因子AB(PDGF-AB)、转化生长因子(TGF-β1)、白介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)浓度。采用单因素方差分析及Bonferroni事后检验比较四种PRP之间的差异。结果四种PRP之间在血小板浓度、富集度和回收率,白细胞浓度、富集度和回收率,以及PDGF-AB、TGF-β1、IL-1β和TNF-α浓度方面的差异均有统计学意义(血小板浓度:F=98.5,P<0.01;血小板富集度:F=253.5,P<0.01;血小板回收率:F=10.8,P<0.01;白细胞浓度:F=124.9,P<0.01;白细胞富集度:F=201.5,P<0.01;白细胞回收率:F=228.4,P<0.01;PDGF-AB浓度:F=139.1,P<0.01;TGF-β1浓度:F=116.2,P<0.01;IL-1β浓度:F=39.3,P<0.01;TNF-α浓度:F=40.6,P<0.01)。Bonferroni事后检验分析提示,威高PRP和Harvest PRP中的血小板和白细胞浓度和富集度以及PDGF-AB、TGF-β1、IL-1β和TNF-α浓度高于Arthrex PRP和Regen PRP(P<0.05),而Regen PRP中的白细胞浓度、富集度以及IL-1β和TNF-α浓度又高于Arthrex PRP(P<0.05)。此外,Bonferroni事后检验分析还提示威高PRP、Harvest PRP以及Regen PRP的血小板和白细胞回收率高于Arthrex PRP(P<0.05)。结论不同套装制备的PRP的细胞和细胞因子成分存在较大差异,临床医师和科研人员合理选择套装,以制备满足其需求的PRP。
Objective To compare the cellular and cytokine components of platelet-rich plasma （PRP） obtained from four commercial preparation systems, so as to provide substantial proof for the commercial PRP preparation systems selection in clinical practice and scientific studies. Methods Between August 2015 and June 2016, the whole blood collected from 20 healthy volunteers were used to prepare PRP with Arthrex, Regen, WEGO, and Harvest PRP preparation systems in a single-donor model. Concentrations of platelets and leukocytes in whole blood and PRP were determined by whole blood analysis. The capture efficiency of platelets or leukcoytes was defined as the quotient of platelet or leukocyte count in PRP divided by platelet or leukocyte count in the whole blood, and the enrichment factor of platelets or leukocytes was defined as the quotient of platelet concentration in PRP divided by platelet or leukocyte concentration in the whole blood. Enzyme-linked immune sorbent assay was applied to quantify platelet-derived growth factor AB （ PDGF-AB）, transforming growth factor β （ TGF-β1）, interleukin-1 （IL-1β） and tumor necrosis factor-α（TNF-α） concentrations in PRP after activation with calcium chloride for 2 h. One-way analysis of variance and Bonferroni post-hoc test were performed to analyze the difference between groups. Results One-way analysis of variance analysis demonstrated that there were significant differences among PRP formulations with respect to platelet concentration, enrichment factor and capture efficiency, leukocyte concentration, enrichment factor and capture efficiency, and concentrations of PDGF- AB, TGF-β1 IL-1β, TNF-α （platelet concentration： F = 98.5, P 〈 0. 01 ; platelet enrichment factor： F = 253.5, P 〈 0. 01 ; platelet capture efficiencies ： F = 10. 8, P 〈 0. 01 ; leukocyte concentration： F = 124. 9, P 〈 0. 01 ; leukocyte enrichment factor： F = 201.5, P 〈 0. 01 ; leukocyte capture efficiency ： F = 228. 4, P 〈 0. 01 ; PDGF-AB concentration： F = 139. 1, P 〈 0. 01 ; TGF-β1 oncentration： F = 116. 2, P 〈0. 01 ; IL-1β ncentratoin： F = 39.3, P 〈 0. 01 ; TNF-αconcentration： F = 40. 6, P 〈 0. 01 ）. Additional Bonferroni post-hoc test showed that the mean concentrations and enrichment rates of platelets and leukocytes, and concentrations of PDGF-AB, TGF-β1, IL-1β, and TNF-α were significantly higher in WEGO PRP and Harvest PRP than Arthrex PRP and Regen PRP （P 〈 0. 05 ）; the mean leukocyte concentration and enrichment rate, and IL-1βand TNF-α concentrations were also significantly higher in Regen PRP than Arthrex PRP （ P 〈 0. 05 ）. Regen PRP, WEGO PRP, and Harvest PRP presented higher platelet and leukocyte capture efficiencies compared with Arthrex PRP （P 〈 0. 05 ）. Conclusion PRP obtained from different commercial preparation systems are different in both cellular and cytokine components ,which will help clinicians and scientists to choose a system that meets their specific needs.
出处
《中华关节外科杂志（电子版）》
CAS
2016年第6期5-9,共5页
Chinese Journal of Joint Surgery(Electronic Edition)
关键词
富血小板血浆
血小板
白细胞
细胞因子
Platelet-rich plasma
Blood platelets
Leukocytes
Cytokines
作者简介
通信作者：王书军，Emai!：docplasticwang@sohu．com
