负性共刺激分子B7-H1与乳腺癌细胞化疗敏感性的关系研究被引量：6

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摘要目的构建B7-H1过表达乳腺癌细胞株,通过观察其在化疗药物表柔比星和多西他赛作用下的增殖和凋亡情况,探讨B7-H1的表达与乳腺癌细胞化疗敏感性的关系。方法利用慢病毒转染MCF-7人乳腺癌细胞株,构建MCF-7/B7-H1^+乳腺癌细胞株和MCF-7/mock乳腺癌细胞株,利用Realtime-PCR、流式细胞术等方法检测细胞株构建情况。进一步采用CCK8法和Annexin-V/PI凋亡试剂盒检测化疗药物表柔比星和多西他赛作用下各组细胞增殖和凋亡情况。结果 MCF-7/B7-H1^+乳腺癌细胞株和MCF-7/mock乳腺癌细胞株构建成功。在化疗药物表柔比星和多西他赛作用下,MCF-7/B7-H1^+细胞相较于MCF-7/mock乳腺癌细胞增殖能力强,细胞凋亡水平低。结论上调B7-H1在MCF-7细胞中的表达降低了化疗药物对MCF-7乳腺癌细胞的增殖抑制能力和凋亡诱导能力。MCF-7/B7-H1^+乳腺癌细胞能够部分抵抗表柔比星和多西他赛的抑制细胞增殖和促进细胞凋亡作用。免疫共刺激分子B7-H1的表达与乳腺癌细胞化疗敏感性相关,上调B7-H1表达可减弱乳腺癌细胞对化疗药物表柔比星和多西他赛的敏感性。

作者刘鸿飞赵帅吴晓鎏蔡长松黄蕾吴恩昊朱旬

机构地区苏州大学附属第二医院普外科

出处《中国临床医生杂志》 2016年第12期69-72,共4页 Chinese Journal For Clinicians

关键词 B7-H1 乳腺癌表柔比星多西他赛化疗敏感性

分类号 R737.9 [医药卫生—肿瘤]

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