摘要
目的观察艾条悬灸对肾阳虚大鼠垂体-肾上腺轴和垂体-甲状腺轴的影响。方法以氢化可的松肌肉注射制作皮质酮肾阳虚动物模型,将大鼠随机分成模型对照组、艾灸治疗组,并设立空白对照组,艾灸治疗组采用艾条悬灸肾俞、关元,每次每穴20 min,每日1次,共治疗14次;治疗结束后采用ELISA方法检测各组大鼠血清CORT、ACTH、T3、T4、TSH的含量,采用RT-PCR方法检测垂体ACTH、TSH m RNA的表达。结果与模型对照组比较,艾灸治疗组血清CORT无显著性差异(P>0.05),血清ACTH、T4、TSH和垂体ACTH、TSH m RNA均具有显著性差异(P<0.05),血清T3具有极显著性差异(P<0.01);与空白对照组比较,艾灸治疗组上述各指标均无显著性差异(P>0.05)。结论艾条悬灸肾俞、关元通过降低血清TSH的含量,下调垂体TSH m RNA的表达和升高血清ACTH、T3、T4的含量,上调垂体ACTH m RNA的表达对肾阳虚大鼠起治疗作用。
Objective To investigate the effect of suspended moxa stick moxibustion on points Shenshu（BL23） and Guanyuan（CV4） on the pituitary-adrenal axis and the pituitary-thyroid axis in rats with kidney yang deficiency. Method A rat model of corticosterone kidney yang deficiency was made by intramuscular injection of hydrocortisone. The rats were randomized into model control and moxibustion treatment groups. A blank control group was set up. The moxibustion treatment group received suspended moxa stick moxibustion on points Shenshu and Guanyuan 20 min once daily, for a total of 14 times. After the completion of treatment, serum CORT, ACTH, T3, T4 and TSH contents were measured by ELISA and pituitary expressions of ACTH and TSH mRNA were determined by RT-PCR. Result There was no significant difference in serum CORT （P〉0.05）, there were significant differences in serum ACTH, T4 and TSH and pituitary ACTH and TSH mRNA （P〈0.05） and there was a very significant difference in serum T3 （P〈0.01） between the moxibustion treatment and model control groups. There were no significant differences in the above indicators between the moxibustion treatment and blank control groups （P〉0.05）. Conclusion Suspended moxa stick moxibustion on points Shenshu and Guanyuan produces a therapeutic effect on rat kidney yang deficiency by decreasing serum TSH content, down-regulating pituitary TSH mRNA expression, increasing serum ACTH, T3 and T4 contents and up-regulating pituitary ACTH mRNA expression.
出处
《上海针灸杂志》
2016年第12期1469-1472,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
江西省教育厅科技项目(GJJ10559)
关键词
艾条灸
肾阳虚
垂体-肾上腺轴
垂体-甲状腺轴
大鼠
Moxa stick moxibustion
Kidney yang deficiency
Pituitary-adrenal axis
Pituitary-thyroid axis
Rats
作者简介
闵友江（1975-），男，副教授，博士
通信作者：程立红（1957-），女，教授，研究方向为刺灸方法与临床研究
