摘要
目的探讨妇科肿瘤患者外周血调节性T细胞(regulatory T cells,Treg)、T细胞亚群表达和血清中转化生长因子β(transforming growth factor-β,TGF-β)、白细胞介素10(interleukin-10,IL-10)细胞因子的变化及相关性。方法选取97例妇科肿瘤患者(宫颈癌患者32例、子宫内膜癌患者35例、卵巢癌患者30例)和健康体检者30例为研究对象,应用流式细胞术检测所有研究对象外周血中CD4^+CD25^+Foxp3^+、CD4^+CD25^+CD127lowTreg占CD4^+T淋巴细胞的比率及T细胞亚群分布;应用ELISA方法检测血清中细胞因子TGF-β1、TGF-β2、IL-10的含量。结果妇科肿瘤组外周血中CD4^+CD25^+Foxp3^+Treg、CD4^+CD25^+CD127lowTreg占CD4^+T淋巴细胞的比率明显高于对照组,差异有统计学意义(P<0.05);T细胞亚群分布与对照组差异无统计学意义(P>0.05);血清中TGF-β1、IL-10含量明显高于对照组,TGF-β2含量低于对照组,差异有统计学意义(P<0.05)。妇科肿瘤组手术1个月后、化疗4个周期后外周血中CD4^+CD25^+Foxp3^+Treg占CD4^+T淋巴细胞的比率,CD4^+CD25^+CD127lowTreg占CD4^+T淋巴细胞的比率,血清中TGF-β1、TGF-β2、IL-10含量及手术1个月后CD4^+T淋巴细胞、CD8^+T淋巴细胞、CD4^+/CD8^+,化疗4个周期后CD4^+、CD19^+T细胞明显低于手术前,差异有统计学意义(P<0.05);CD4^+CD25^+Foxp3^+Treg占CD4^+T淋巴细胞的比率与血清TGF-β1含量之间存在正相关(rs=0.220,P=0.034);CD4^+CD25^+CD127low Treg占CD4^+T淋巴细胞的比率与血清中TGF-β1、TGF-β2、IL-10含量之间均存在正相关(rs=0.284、0.385、0.336,P<0.01)。结论妇科肿瘤患者其免疫耐受机制可能与CD4^+CD25^+Foxp3^+Treg、CD4^+CD25^+CD127lowTreg占CD4^+T淋巴细胞的比率及细胞因子TGF-β1、TGF-β2、IL-10含量相关。
Objective To explore the expression of regulatory T cells（ Treg） and T cell subgroup in peripheral blood and the variation and correlation of transforming growth factor-β（ TGF-β） and interleukin-10（ IL-10） cytokines in serum in patients with gynecological cancer. Methods Ninety-seven cases（ 32 cases with cervical cancer,35 cases with endometrial cancer,30 cases with ovarian cancer） of patients with gynecological cancer and 30 cases of healthy controls were obtained as research objects.Flow cytometry was used to detect CD4^＋CD25^＋Foxp3^＋and CD4^＋CD25^＋CD127lowregulatory T cells and the distribution of T cell subgroup in peripheral blood of all the subjects. ELISA was used to detect the content of TGF-β1、TGF-β2 and IL-10 cytokines in serum. Results Compared with healthy controlgroup,CD4^＋CD25^＋Foxp3^＋and CD4^＋CD25^＋CD127lowregulatory T cells accounted for the proportion of CD4^＋T cells expression in patients with gynecological cancer group in peripheral blood were significantly higher（ P〈0. 05）. There was no statistical significant difference in T cell subgroup proportion（ P〈0. 05）. The contents of TGF-β1and IL-10 in serum were significantly higher（ P〈0. 05）,the content of TGF-β2 was significantly lower（ P〈0. 05）. The proportion of CD4^＋CD25^＋Foxp3^＋and CD4^＋CD25^＋CD127lowregulatory T cells accounted for CD4^＋T cells in peripheral blood and TGF-β1、TGF-β2 and IL-10 in serum expression in patients with gynecological cancer group were significantly lower in one month after the operation,and after four cycles of chemotherapy（ P〈0. 05）,CD4^＋、CD8^＋T cells and CD4^＋/CD8^＋in one month after the operation、CD4^＋、CD19^＋T cells after four cycles of chemotherapy were significantly lower than that before the operation（ P〈0. 05）. There was positive correlation between CD4^＋CD25^＋Foxp3^＋Treg and TGF-β1（ rs= 0. 220,P = 0. 034）. There were positive correlation between CD4^＋CD25^＋CD127lowregulatory T cells and the content of TGF-β1、TGF-β2 and IL-10（ rs= 0. 284,0. 385,0. 336,P〈0. 01）. Conclusion The immune tolerance mechanism in patients with gynecology cancer might be related to the ratio of CD4^＋CD25^＋Foxp3^＋Treg and CD4^＋CD25^＋CD127lowTreg accounted to the proportion of CD4^＋T cells,and to the content of TGF-β1、TGF-β2 and IL-10 cytokines.
出处
《河北医科大学学报》
CAS
2016年第11期1307-1311,1316,共6页
Journal of Hebei Medical University
基金
秦皇岛市科学技术研究与发展计划(20141A219)
作者简介
[作者简介]于丹军（1973-），男，辽宁建昌人，河北省秦皇岛市第-医院副主任技师，医学硕士，从事肿瘤免疫学研究。
