摘要
铬是植物的非必需元素,对植物的生长发育具有显著的抑制作用.采用双向电泳和质谱技术,研究了铬胁迫对西红花叶片蛋白表达的影响.通过质谱技术,成功鉴定出9个在铬胁迫后下调表达的蛋白,分别为细胞分裂循环蛋白48、ATP合成酶α亚基、核酮糖二磷酸羧化酶长链(2个)、未知蛋白、核酮糖二磷酸羧化加氧酶、蛋白酶体α亚基、铁蛋白和蛋白酶体β亚基;6个上调表达的蛋白,分别为蔗糖合酶、真核起始因子4A、α-1,4-葡聚糖蛋白合成酶、1-氨基环丙烷-1-羧酸氧化酶、异黄酮还原酶类似物IRL和未知蛋白.以上结果为研究植物响应铬胁迫的分子机制提供了参考.
Chromium（Cr）is a nonessential element for plants.Cr not only affects a series of physiological processes in plants,but also significantly inhibits the growth and development of plants.Under Cr stress,plants have evolved complex detoxification mechanisms.In the present study,we investigated the protein expression of leaves of saffron in response to Cr stress via 2Delectrophoresis and mass spectrum analysis.Nine proteins were identified to be down-regulated under Cr stress,including cell division cycle protein 48 homolog,ATP synthase subunit alpha,ribulose bisphosphate carboxylase large chain,Proteasome subunit alpha type,etc.Six proteins were up-regulated,including sucrose synthase 2,eukaryotic initiation factor 4A,Alpha-1,4-glucan-protein synthase,1-aminocyclopropane-1-carboxylate oxidase and Isoflavone reductase homolog IRL,etc.The results provide valuable insights towards the molecular mechanism of saffron in response to Cr toxicity.
出处
《浙江大学学报（理学版）》
CAS
CSCD
北大核心
2016年第6期751-755,共5页
Journal of Zhejiang University（Science Edition）
基金
科技部国家星火计划项目(2015GA700053)
浙江省自然科学基金资助项目(LY13B020001)
浙江省科技厅公益技术研究农业项目(2015C32104)
关键词
铬
西红花
叶片
蛋白组
chromium
saffron
leaf
proteomics
作者简介
饶君风（1969-），ORCID：http：//orcid．org／0000—0000—0002—069X，女，副教授，主要从事中药品质评价和资源应用研究E—mail：13588867756@139．com．
