摘要
目的研究黑木耳多糖（AAP）和松多酚（PKP）联合使用对^（60）Coγ辐射诱导小鼠氧化损伤的修复作用。方法昆明小鼠除对照组外^（60）Coγ射线一次性全身均匀照射,总吸收剂量为4.0 Gy,吸收剂量率为1.0 Gy/min。辐射24 h后分别设AAP组、PKP组、AAP与PKP协同（AP）组及茶多酚（TP）组,分别ig 75、25、37.5＋12.5、25 mg/kg（以多糖或多酚水平计）相应药物,对照组和模型组ig等体积生理盐水。连续喂养30 d后空腹24 h处死,测定小鼠的血清、脾脏、肝脏、心脏中维生素C（VC）、谷胱甘肽（GSH）、辅酶Q10（Co Q10）、丙二醛（MDA）水平和超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性;取小鼠无菌脾细胞进行彗星电泳,荧光显微镜成像系统采集图像。结果辐射后给药PKP、AAP、AP和TP均可一定程度上升高VC、GSH、Co Q10水平,升高SOD、GSH-Px活性,降低MDA水平,减小脾细胞彗星尾长度,其中AP组辐射后修复效果较好。结论与AAP、PKP、TP组比较,AP不仅可以促进体质量的恢复,还能有效地清除体内自由基,降低自由基对体内DNA造成的损伤,显著提高^（60）Coγ辐射诱导氧化损伤的修复能力。
Objective To study the effect of polysaccharides from Auricularia auricula（AAP） and polyphenols from Pinus koraiensis（PKP） combinations（AP） on recoveries of antioxidant injury induced by ^（60）Coγ radiation. Methods The model group and five experimental groups were irradiated once with ^（60）Coγ ray. AAP, PKP, AP, and TP had been given to mice by gavage for 30 d at doses of 75.0, 25.0, 37.5＋12.5 and 25.0 mg/kg（calculate with polysaccharide or polyphenol levels）, respectively. The mice in control and model groups were given normal saline by gavage during that time. After 30 d, contents of vitamin C（VC）, glutathione（GSH）, coenzyme Q10（Co Q10）, and malondialdehyde（MDA）, and activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in the serum, spleen, liver, and heart of mice were determined. Meanwhile, the nuclear DNA degradation in spleen was observed by comet assay. Results It showed that AAP, PKP, AP, and TP could significantly increase the contents of VC, GSH, Co Q10, improve the activities of SOD and GSH-Px, reduce MDA content, and decrease the comet tail lengths as compared to model group. Above all, effect of AP on antioxidant recovery was the best. Conclusion Compared to AAP, PKP, and TP groups, AP not only can faster pick up flesh, but also more effectively remove free radicals and reduce damages to DNA, more significantly improve antioxidant recovery from radiation induced oxidative injury.
作者
张乃珣
尹红力
赵鑫
刘冉
王振宇
ZHANG Nai-xun YIN Hong-li ZHAO Xinj LIU Ran WANG Zhen-yu(College of Forestry, Northeast Forest University, Harbin 150040, China Institute of Food Science and Technology CAAS/Institute of Staple Food Processing Technology, Harbin 151900, China Liaocheng University, Agricultral College, Liaocheng 252000, China Department of Food Science and Engineering,Harbin Institute of Technology, Harbin 150090, China)
出处
《药物评价研究》
CAS
2016年第5期722-729,共8页
Drug Evaluation Research
基金
国家自然科学基金资助项目(31170510)
中央高校基本科研业务费专项(DL11BA18)
关键词
黑木耳多糖
松多酚
茶多酚
协同
辐射
氧化损伤
修复
polysaccharides from Auricular auricular
polyphenols from Pinus koraiensis
tea polyphenols
synergy
radiation
oxidative injury
recovery
作者简介
张乃珣（1991-），女，在读博士研究生，主要研究方向为植物资源开发利用。E-mail：zhangnaixun66@163．com
通信作者王振宇（1957-），男，研究生导师，教授，主要研究方向为植物资源开发利用。E-mail：wzy219001@163．com
