摘要
[目的]了解白蜡虫泌蜡高峰期所表达的热激蛋白及其序列特征。[方法]从白蜡虫转录组数据库筛选获得hsp10、hsp20、hsp40、hsp60、hsp70、hsp90基因序列,进行序列联配和进化树分析,并对hsp40、hsp60、hsp90基因在不同温度下mRNA相对表达量情况进行荧光定量PCR检测。[结果]从白蜡虫转录组数据库中共筛选出32条hsp基因序列,序列分析发现它们与目前已报道的序列具有很高一致性,大部分含有保守区域和特征序列,能够与同目昆虫基因聚为一支。hsp40、hsp60、hsp90基因均能被温度诱导。[结论]在白蜡虫泌蜡高峰期共鉴定到32个热激蛋白非重复序列基因(unigene):hsp10 unigene 3个,hsp20 unigene 1个,hsp40 unigene 11个,hsp60 unigene 1个,hsp70 unigene 13个,hsp90 unigene 3个。
[ Objective ] The study aims at investigating the heat shock protein （hsp） genes and analyze the sequence characterization of Ericerus pela during the peak period of wax-secreting. [ Method ] The sequences of hsplO, hsp20, hsp40, hsp60, hspTO, and hsp90 were obtained from the transcriptome database of E. pela. The sequence a- lignment and phylogenetic analysis were performed. And the mRNA expression patterns of hsp40, hsp60, and hsp90 under different temperature treatments were carried out by using the quantitative real time PCR （qRT-PCR）. [ Re- sult] A total of 32 HSPs, which were highly similar with the HSP reported in other species, were identified. Most of them had the conserved regions and characteristic sequences, and they were clustered together with the sequences of the same order insect in the phylogenetic trees. The qRT-PCR results showed that, hsp40, hsp60, and hsp90 can be induced by temperature-stress. [ Conclusion] In this study, 32 unigenes of HSP of E. pela were identified, inclu- ding 3 hspl0 unigenes, 1 hsp20 unigenes, 11 hsp40 unigenes, 1 hsp60 unigenes, 13 hspTO unigenes, and 3 hsp90 unigenes.
出处
《林业科学研究》
CSCD
北大核心
2016年第5期778-783,共6页
Forest Research
基金
国家863计划2014AA021801
林业公益性行业科研专项201504302、201304808
云南省应用基础研究重点项目2013FA052
国家自然科学基金31572337
关键词
白蜡虫
热激蛋白
序列分析
温度胁迫
荧光定量PCR
Ericerus pela
heat shock protein
sequence analysis
temperature-stress
quantitative real time PCR
作者简介
孙涛（1991-），男，硕士研究生，主要从事昆虫分子生物学研究．
通讯作者：杨璞
