摘要
为探究真菌群落在凋落物分解进程中的影响,以在油松人工林中分离纯化而来的3株真菌Irpex lacteus(白囊耙齿菌)、Collybia subnuda(金钱菌属)和Trametes hirsuta(毛栓菌)为供试菌株,经组合形成不同真菌群落,以油松针叶、蒙古栎阔叶凋落物及两者混合形成3种类型分解基质,通过发酵纯培养的方法,测定了底物有机质量损失及发酵过程中羧甲基纤维素酶(CMCase)活性变化,并验证了酶活性与底物降解的关系。结果表明:凋落物类型和真菌组合均显著影响凋落物分解,且两者之间存在交互作用;真菌多样性提高了凋落物的分解速率;凋落物混合产生了正面非加性效应,且这种效应主要由阔叶引起。质量损失率和纤维素酶活性呈现类似的规律,即同一凋落物类型下,组合菌群大于或等于单一菌群;同一真菌群落下,阔叶>混合>针叶。
To explore the effect of the fungal communities in the process of litter decomposition, three strains of fungus, Irpex lacteus, Collybia subnuda and Trametes hirsuta separated and puriifed from Chinese Pine Plantations were as the tested strains, which Combined to form different fungal communities, pinus tabulaeformis coniferous and broad-leaved litter Mongolian oak and the mixed to form three types of zymolyte, the method of fermentation was used to assay the mass defect of zymolyte and the activies of carboxymethyl cellulase （CMCase） in fermenting process, and try to comifrm the relationship between the activities of enzyme and the mass defect of zymolyte. The results showed that the litter types and fungus were signiifcantly affect the litter decomposition, and there was interaction between them;Fungal diversity improves the rate of litter decomposition;Litter mixture produced positive than additive effect, and this effect is mainly caused by broadleaf. Mass loss and cellulase activity present a similar rule, namely under the same litter type, combination lfora is greater than or equal to a single lfora;under the same fungal community, broad-leaved〉mixed〉needles.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2016年第7期41-47,共7页
Journal of Central South University of Forestry & Technology
基金
国家自然科学基金项目(30972353)
作者简介
杨晨，硕士研究生
通讯作者：刘勇，教授，博士，博士生导师；E-mail：lyong@bjfu．edu．cn
