摘要
为探讨内生真菌在凋落叶分解过程中的作用,将自杉木分离纯化的3株真菌青霉属(Penicillium)CG2 (A菌)、黄色镰刀菌(Fusarium culmorum)AY13(B菌)和踝节霉菌(Talaromyces)AJ14(C菌)作为分解菌株,以菌丝、灭菌发酵液以及单菌和混菌的方式添加到装有杉木凋落叶的盆钵中,同时设置未添加真菌的对照(CK)处理,研究杉木凋落叶在不同菌株调控下10 d、30 d、60 d、90 d以及120 d内的分解动态.结果显示,A菌液处理在120 d内凋落叶质量损失率最高,而AC菌丝处理60 d时与对照存在显著差异(P <0.05),比对照高23.97%. 60 d时凋落叶碳含量在A菌丝处理下与对照有显著差异(P <0.05),比对照低16.74%,而B菌丝处理90 d时含量最低,比对照低21.13%;大部分菌丝、菌液处理下氮含量较对照增加,其中A菌液处理下与对照有显著差异(P <0.05),比对照高17.05%;与氮元素相似,磷含量同样增加,A菌丝处理与对照有显著差异,比对照高46.67%;而钾含量在C菌液处理下比对照低28%,与对照存在显著差异(P <0.05);90 d时碳氮比在A菌丝和B菌丝处理下数值最低,分别比对照低25.54%和25.11%,且都与对照有显著差异(P <0.05),而A菌丝处理60 d时碳磷比最低,与对照有显著差异(P <0.05),比对照低43.05%.上述结果表明,3株内生真菌对杉木凋落叶质量损失率、养分含量的影响不同,A菌株影响明显,这对杉木菌肥的选育有参考价值.
To explore the role of endophytic fungi in the decomposition of litter, the endophytic fungi Penicillium sp. strain CG2 (A), Fusarium flavum strain AY13 (B), and Talaromyces strain AJ14 (C) of Cunninghamia lanceolata were added to experimental pots in different forms (mycelium, sterilized fermentation broth, single fungus, and mixed fungi), and a control treatment (CK) was set up (no fungi added). At 10, 30, 60, 90, and 120 days after litter decomposition, a study on the decomposition dynamics of C. lanceolata litter under different treatments was performed. The results showed that the rate of leaf mass loss was the highest in the sterilized fermentation broth treatment A after 120 days, and that there was a significant difference (P<0.05) between the mycelium treatment AC and the control treatment after 60 days (23.97% higher than the control group). On day 60, the litter carbon content from the mycelium treatment A was significantly different from that of the control (P<0.05), showing a 16.74% lower value, whereas the litter carbon content of the mycelium treatment B was 21.13% lower than that of the control after 90 days. The nitrogen content of the litters of most mycelium and sterilized fermentation broth treatments was increased compared to that of the control group;there was significant difference (P<0.05) between the sterilized fermentation broth treatment A and the control (P<0.05), with a 17.05% higher value than that of the control. Similar to nitrogen, the litter phosphorus content also increased;there was a statistically significant difference between the mycelium treatment A and the control group, with treatment A showing a 46.67% higher value than the control group. The potassium content was 28% lower than that of the control group under the sterilized fermentation broth treatment C, a result that was significantly different from that of the control group (P<0.05). After treatment for 90 days, the ratio of carbon to nitrogen was the lowest under the treatments with the mycelium A and the mycelium B, with values 25.54% and 25.11% lower than that of the control group, respectively, and a statistically significant difference from that of the control group (P<0.05). The ratio of carbon to phosphorus was the lowest under the treatment with mycelium A after 60 days, and the result was significantly different from that of the control (P<0.05), with a 43.05% lower value than the control. Thus, the three endophytic fungi had different effects on the mass loss rate and nutrient content of the litter. The Penicillium sp. strain CG2 (A) had statistically significant effects on the mass loss and nutrient content of?leaf litter, which was within the range of fungi fertilizer reference values for the breeding of C. lanceolata.
作者
李冠军
梁安洁
洪滔
林勇明
吴承祯
洪伟
林晗
李键
LI Guanjun;LIANG Anjie;HONG Tao;LIN Yongming;WU Chengzhen;HONG Wei;LIN Han;LI Jian(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Ecology and Resource Engineering,Wuyi University,Nanping 354300,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2018年第6期1211-1220,共10页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31400533)
福建省自然科学基金项目(2014J01074)
福建农林大学科技创新专项基金项目(CXZX2016055)
福建农林大学林学院林学高峰学科优秀博硕士培养工程项目(71201800781)资助~~
关键词
杉木
凋落叶
内生真菌
质量损失率
养分含量
化学计量比
Cunninghamia lanceolate
leaf litter
endogenous fungi
mass loss rate
nutrient content
stoichiometric ratio
作者简介
共同通讯作者:吴承祯,(E—mail:fjwcz@126.com);共同通讯作者:李键,(E-mail:hmilycau@163.com).
