摘要
目的:银桑菊固体饮料的质量标准,建立其HPLC指纹图谱。方法:采用HPLC法对10批银桑菊固体饮料样品进行分析,以Kromasil C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-0.4%磷酸溶液为流动相,梯度洗脱,检测波长268 nm,流速1.0 m L·min-1,柱温35℃,进样量10μL;利用国家药典委员会"中药色谱指纹图谱相似度评价系统"(2.0版)标定共有峰和相似度评价。结果:建立了银桑菊固体饮料的HPLC指纹图谱,标定了20个共有峰,10批样品的相似度均>0.9,并指认了3个峰分别为绿原酸、甘草苷、木犀草苷。结论:该法操作简便可靠,所建立的HPLC指纹图谱重复性良好,可用于银桑菊固体饮料的质量评价。
Objective： To establish the HPLC fingerprint of Yinsangju solid beverage. Method： Ten batches of Yinsangju solid beverage were analyzed by HPLC method on Kromasil C18column（ 4. 6 mm × 250 mm,5 μm） with acetonitrile- 0. 4% phosphoric acid solution as the mobile phase for gradient elution. The detection wavelength was 268 nm; flow rate was 1. 0 m L·min- 1; column temperature was 35 ℃ and the injection volume was10 μL. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（ version2. 0） was used to identify common peaks and evaluate similarity. Result： The HPLC fingerprint of Yinsangju solid beverage was established. Twenty common peaks were identified with similarity of more than 0. 9 for 10 batches of samples. Three peaks were identified as chlorogenic acid,liquiritin,and luteolin glycosides. Conclusion： This reliable,available and quick method can be used in the quality evaluation of Yinsangju solid beverage.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第18期68-71,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重庆市科技支撑示范工程项目(cstc2014fqzktjcsf10019)
关键词
银桑菊固体饮料
指纹图谱
绿原酸
甘草苷
木犀草苷
Yinsangju solid beverage
fingerprint
chlorogenic acid
liquiritin
luteolin glycosides
作者简介
李跃辉，副研究员，从事中药制剂与分析研究，Tel：13755056665，E—mail：410256518@qq．com
[通讯作者]向丽，中药师，从事中药饮片质量控制及研发，Tel：18523532313，E—mail：410256518@qq．com
