摘要
[目的]建立陕西产延胡索药材的高效液相色谱(HPLC)指纹图谱,为延胡索药材制定质量标准提供参考。[方法]采用反相高效液相色谱法,KromasilC18色谱柱,柱温30℃,流动相:乙腈—醋酸盐缓冲液(pH6.0)梯度洗脱,流速1.0mL/min,检测波长280nm,洗脱时间75min。[结果]建立了以延胡索乙素为内参照峰的延胡索药材HPLC指纹图谱,共含有17个共有色谱峰。[结论]建立的指纹图谱可以作为延胡索质量评价和品种鉴别的主要依据之一。
[Objective] To set up a method for HPLC-fingerprint of Rhizoma Corydalis in Shanxi. The conclusion makes reference to standard of quality control of Rhizoma Corydalis. [Methods] The methods were performed by RP-HPLC. Kromasil ODS-C18 Column was used at 30 ℃, acetonitrile-acetate buffer solution (pH 6.0) as mobile phase, gradient eluting, flow rate was 1.0 mL/min, detection wavelength was set at 280 nm and eluting period were 75 min. [Results] HPLC-fingerprint of Rhizoma Corydalis was established, which contained 17 common peaks. [Conclusions] The established fingerprint can be used for the quality control and species identifying of Rhizoma Corydalis.
出处
《天津中医药大学学报》
CAS
2007年第3期150-153,共4页
Journal of Tianjin University of Traditional Chinese Medicine
基金
国家自然基金面上项目(30672664)
天津市高等学校科技发展基金项目(20040309)。
作者简介
窦志英(1967-),女,副教授,研究方向为中药有效成分研究及中药炮制.
