摘要
目的 建立黄连上清片中盐酸小檗碱、盐酸巴马汀、盐酸药根碱、表小檗碱和黄连碱的测定方法。方法 采用反相高效液相色谱法。色谱柱为Agel C18反相柱,流动相为乙腈-2.5mmo L·L-1庚烷磺酸钠和50mmo L·L-1磷酸二氢钾溶液（含0.14%三乙胺,磷酸调p H至3.0）=27∶73,检测波长为348nm,柱温为35℃,流速为1.0m L·min-1,进样量为10μL。结果 5种生物碱成分色谱分离行为良好,盐酸小檗碱检测浓度在10.14~101.40μg·m L-1范围内与峰面积积分值呈良好线性关系,平均加样回收率为98.88%,RSD=0.74%;盐酸巴马汀检测浓度在4.53~45.30μg·m L-1范围内与峰面积积分值呈良好线性关系,平均加样回收率为99.12%,RSD=0.69%;盐酸药根碱检测浓度在1.54~15.40μg·m L-1范围内与峰面积积分值呈良好线性关系,平均加样回收率为98.07%,RSD=1.03%;表小檗碱检测浓度在3.09~30.90μg·m L-1范围内与峰面积积分值呈良好线性关系,平均加样回收率为99.79%,RSD=1.03%;黄连碱检测浓度在4.56~45.60μg·m L-1范围内与峰面积积分值呈良好线性关系,平均加样回收率为98.50%,RSD=0.89%。结论 本方法灵敏度高,专属性强,准确性好,重复性好,可用于黄连上清片的质量控制。
OBJECTIVE To establish the HPLC method for the content determination of berberine hydrochloride, palmatine hydroehloride, jatrorrhizine hydrochloridein, epiberberine and coptisine in Huanglian shangqing tablets. METHODS RP-HPLC method was adopted. The determination was performed on Agel C18 column with mobile phase consisted of aeetonitrile-2.5mmoL·L-1 sodium heptanesulfonate-50mmoL·L-1 monopotassium phosphate （including 0. 14% triethylamine,pH value adjusted to 3.0 by phosphoric acid, 27：73 ） at a flow rate of 1.0mL ·min-1. The determination wavelength was set at 348nm and column temperature was 35℃. The injection volume was 10μL. RESULTS The linear range of berberine hydrochloride was 10. 14 - 101. 40μg·mL-1 ,with average recovery of 98.88% （ RSD =0. 74% ）. The linear range of palmatine hydroehloride was 4. 53 -45.30μg·mL-1 with average recovery of 99. 12% （RSD =0. 69% ）. The linear range of jatrorrhizine hydrochloridein was 1.54 - 15.40μg·mL-1 with average recovery of 98.07% （ RSD = 1.03% ）. The linear range of epiberberine was 3.09 - 30. 90μg·mL-1 with average recovery of 99. 79% （ RSD = 1.03% ）. The linear range of coptisine was 4. 56 -45.60μg·mL-1 with average recovery of 98.50% （RSD = 0. 89% ）. CONCLUSION The method is sensitive, accurate, specific and reproducible for content determination of berbeberberine hydrochloride, palrnatine hydrochloride, jatrorrhizine hydrochloride, epiberberine and coptisine in in Huanglian shangqin tablet.
出处
《海峡药学》
2016年第8期74-77,共4页
Strait Pharmaceutical Journal
关键词
盐酸小檗碱
盐酸巴马汀
盐酸药根碱
表小檗碱
黄连碱
高效液相色谱法
黄连上清片
含量测定
Berberine hydrochloride
Palmatine hydrochloride
Jatrorrhizine hydrochloridein
Epiberberine
Coptisine
HPLC
Huanglian shangqing tablet
Content
作者简介
胡紫艳，女（1982．09-）。硕士，毕业于中国药科大学，职称：助理研究员。研究方向为药物分析。E—mail：huzy0903@163．com
