摘要
目的:测定黄连药材及其炮制品中盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的含量,制定2010年版《中国药典》中黄连及其炮制品的质量标准。方法:以十八烷基硅烷键合硅胶为填充剂;流动相为乙腈-50mmol/L磷酸二氢钾溶液(50∶50)(混合液中加15mmol/L十二烷基硫酸钠,再以磷酸调节pH为4.0);流速:0.6mL/min;检测波长:345nm;柱温:30℃。结果:黄连药材中盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的含量分别不得少于0.93%、1.80%、1.45%、6.00%;黄连饮片、酒黄连、姜黄连和萸黄连中盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱含量分别不得少于0.70%、1.50%、1.10%和5.00%。结论:该方法简便、灵敏、重现性好,适用于黄连及其炮制品质量品质的控制。
AIM： The contents of epiberberine hydrochloride,coptisine hydrochloride,palmatine hydrochloride,and berberine hydrochloride in the crude and processed Rhizoma Coptidis were determined to establish their quality standards for editing Chinese pharmacopoeia of 2010 edition. METHODS： The C18 column was used,the mobile phase was acetonitrile-50 mmol/L monopotassium phosphate （50 ∶ 50）（containing 15 mmol/L SDS and adjusting pH = 4. 0 with phosphoric acid） at the flow rate of 0. 6 mL/min,the detective wavelength was set up at 345 nm and the column temperature was 30 ℃. RESULTS： The contents of epiberberine hydrochloride,coptisine hydrochloride,palmatine hydrochloride,and berberine hydrochloride in Coptidis above 0. 93% ,1. 80% ,1. 45% and 6. 00% ,respectively. The contents of epiberberine hydrochloride,coptisine hydrochloride,palmatine hydro-chloride,and berberine hydrochloride in Rhizoma Coptidis decoctin pieces,Rhizoma Coptidis processed with wine, Rhizoma Coptidis processed with ginger and Rhizoma Coptidis processed with Fructus Evodia above 0. 70% , 1. 50% ,1. 10% ,5. 00% ,respectively were of the utmost importance. CONCLUSION： The method is simple and accurate,and can be used to control the quality of the curde and processed Rhizoma Coptidis.
出处
《中成药》
CAS
CSCD
北大核心
2010年第9期1540-1544,共5页
Chinese Traditional Patent Medicine
基金
国家科技支撑计划项目-川产道地药材川贝母
白芷
黄连的系统研究与开发(2007BAI40B03)
<中国药典>2010版姜黄连炮制品标准制定(Yz-252)
<中国药典>2010版酒黄连炮制品标准制定(Yz-251)
<中国药典>2010版萸黄连炮制品标准制定(Yz-253)
<中国药典>2010版黄连饮片标准制定
作者简介
作者简介：阳勇（1979-），男，助理研究员，主要从事中药化学研究。E-mail：yangychem@126．com
通讯作者：罗维早（1962-），男，研究员，主要从事中药化学及新剂型方向的研究工作。Tel：（023）89029026E—mail：loweizao@163．com
