摘要
在我们先前的研究中发现人参非皂苷部分具有改善小鼠记忆活性。为确定活性组分,实验采用对人参水提物经超滤、透析、Sephadex G-15凝胶柱层析分离纯化,通过UV对糖与蛋白的监测,得到P1、P2两部分。药理实验结果表明,P2经腹腔注射给药7 d,40 mg/kg,能明显改善小鼠记忆获得障碍和小鼠记忆巩固障碍。P2采用纳升级超高效液相-质谱联用仪(Nano LC-MS/MS)进行结构分析,首次从人参中得到22种新肽,且具有辅助改善学习记忆的作用。
Our previous study showed that Pana~ ginseng aqueous extract showed memory-enhancement effect on mice. In this study, two ginseng components （named P1 and P2） were separated from the aqueous extract of P. ginseng through uhrafiltration, dialysis and Sephadex G-15 column chromatography. Their memory-enhancement effect was tested by Morris water maze and step-down experiments. The results of pharmacologic experiment showed that P2 improved the dis- turbance of memory acquisition and consolidation in mice by i. p. at the dose of 40 mg/kg for 7 days. Hence, P2 was the active site of the ginseng aqueous extract with memory-enhancement effect. The structural identification of P2 by nano-ul- tra performance liquid chromatography coupled to LTQ Orbitmp Elite mass spectrometry （Nano LC-MS/MS） was under- taken,the MS/MS ion spectra were evaluated through Uniprot all species sequence database and Proteome Discoverer software, then 22 novel peptides were firstly identified from P. ginseng.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第8期1223-1227,1232,共6页
Natural Product Research and Development
基金
国家自然科学基金(81473326)
吉林省卫生计生青年科研课题(2014Q011)
作者简介
通讯作者Tel：86-431-86058667：E-mail：wangying02231@sina．com
