摘要
角鲨烯含量是高品质植物食用油评判标准的重要指标之一。鲨烯合酶是角鲨烯合成直接相关的上游调控关键酶。本研究在已构建的油茶种仁转录组数据库基础上,设计特异引物,采用RACE技术获得油茶鲨烯合酶基因的全长cDNA序列,命名为CoSQS(Gen Bank登录号为JX914592)。生物信息学分析结果表明:该序列全长1554 bp,其中含全长的开放阅读框为1245 bp,编码415个氨基酸残基,CoSQS蛋白为弱碱性非分泌型蛋白,具有2个明显的跨膜区和2个角鲨烯和番茄红素合成酶的特异信号区。与柿Dk SQS同源蛋白亲缘关系最近,属于疏水性蛋白。亚细胞定位试验结果显示该基因定位于叶绿体。实时荧光定量PCR分析表明,5-10月间,油茶种仁中CoSQS基因表达量呈现先上升后下降的趋势,转录最高峰在9月下旬。通过关联分析表明CoSQS基因表达量与角鲨烯含量密切相关。
Squalene content is one of the evaluation standards for high quality edible oil. Squalene synthase is the directly upstream regulation key enzyme for squalene synthesis. In this study,based on the transcriptome database of Camellia oleifera seed kernel,the specific primers were designed and full-length cDNA sequence was obtained by RACE method. The c DNA was named as CoSQS（ Gen Bank accession No.JX914592）. The results of bioinformatics analysis showed that the full-length cDNA was 1554 bp with a 1245 bp open reading frame. The gene encoded 415 amino acid residues. CoSQS protein was alkalescent non-secreted protein with two transmembrane regions and two specific signal area of squalene and phytoene synthase.CoSQS protein had the closest relationship with Diospyros kaki Dk SQS and belonged to a hydrophobic protein.Real time fluorescent quantitative PCR analysis showed that during May to October,the expression of CoSQS was firstly up-regulated and then down-regulated. The transcription peak was in late September. CoSQS gene expression was closely related with squalene content.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2016年第4期748-755,共8页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(31500556)
湖南省自然科学基金项目(14JJ2104)
关键词
油茶
鲨烯合酶
分子信息
表达分析
Camellia oleifera
squalene synthetase
molecular characterization
expression analysis
作者简介
第一作者主要从事经济林栽培育种及林业生物技术研究。E-mail：zengyanling110@126．com
