摘要
背景:对肿瘤干细胞相关基因ABCE1的深入研究有可能会发现它在参与恶性肿瘤的发生及进展等方面的重要作用。目的:观察沉默ABCE1基因电转染卵巢癌肿瘤干细胞的增殖特性及对β-榄香烯乳的敏感性。方法:设计合成ABCE1的siR NA序列,通过电转染入卵巢癌肿瘤干细胞。RT-PCR和Western blot检测ABCE1基因沉默后卵巢癌肿瘤干细胞ABCE1 m RNA及蛋白的表达,流式细胞仪检测细胞周期变化,MTT法和细胞克隆形成实验分析ABCE1基因沉默后卵巢癌肿瘤干细胞对β-榄香烯乳的敏感性。结果与结论:(1)ABCE1基因沉默后卵巢癌肿瘤干细胞的ABCE1基因和蛋白表达明显降低;(2)ABCE1基因沉默后细胞周期被阻滞在G0/G1期,S期细胞数减少;(3)ABCE1基因沉默后β-榄香烯乳对卵巢癌肿瘤干细胞的增殖抑制率和克隆形成抑制率显著增加;(4)结果表明,沉默ABCE1基因能显著抑制卵巢癌肿瘤干细胞的增殖能力,并能增强肿瘤干细胞对β-榄香烯乳的敏感性。
BACKGROUND：It is likely to find that ABCE1 plays an important part in the occurrence and progression of cancers through in-depth study. OBJECTIVE：To investigate the effects of ABCE1 silencing on the proliferation and sensitivity toβ-eiemene of ovarian cancer stem cel s via electrotransfection. METHODS：siRNA sequence of ABCE1 was designed and synthesized, and then was electrotransfection into ovarian cancer stem cel s. Subsequently, expressions of ABCE1 mRNA and protein in ovarian cancer stem cel s after ABCE1 silencing were detected by RT-PCR and western blot assay;the cel cycle was detected using flow cytometry;the sensitivity of ovarian cancer stem cel s toβ-eiemene after ABCE1 silencing was analyzed by MTT assay and colony-formation assay. RESULTS AND CONCLUSION：After ABCE1 silencing, expressions of ABCE1 mRNA and protein in ovarian cancer stem cel s were significantly reduced. And the cel cycle was arrested in G0/G1 phase, and the number of cel s in S phase significantly decreased. Furthermore, the sensitivity of ovarian cancer stem cel s toβ-eiemene after ABCE1 silencing was significantly enhanced. In conclusion, ABCE1 silencing cannot only significantly inhibit the proliferation of ovarian cancer stem cel s, but also enhance the sensitivity of cancer stem cel s toβ-eiemene.
出处
《中国组织工程研究》
CAS
北大核心
2016年第28期4149-4154,共6页
Chinese Journal of Tissue Engineering Research
作者简介
王俊，男，1979年生，湖北省黄石市人，汉族，2004年成宁医学院毕业，主治医师，主要从事肿瘤及肿瘤疾病相关学科研究。
