摘要
雄烯二酮的生物转化过程受到较多因素的制约.通过响应面优化耐底物突变株MN4培养基的主要成分以提高转化过程中AD的产量.在摇瓶培养条件下,通过Plackett-Burman实验设计发现玉米浆、Na H2PO4、豆油是菌株MN4降解植物甾醇产生雄烯二酮的主要因素.采用最陡爬路径逼近最大响应面区域,然后利用响应面法进行回归分析,对分支杆菌转化成植物甾醇生成雄烯二酮的培养基进行优化,确定最佳培养基组成.实验分析表明,转化培养基的最佳组成为:玉米浆2%、Na H2PO40.07%、豆油14.49%.利用该培养基进行发酵转化验证实验,雄烯二酮平均生产量达到6.23 g·L-1,生物转化率为55.3%,比原始生成水平(4.65 g·L-1)提高34%.
Biotransformation processing of androstenedione is affected by many factors. Response surface methodology was applied to optimize the medium compositionfor enhancing the androstenedione production by substrate resistance mutant strains MN4. Corn steep liquor,Na H2PO4 and soybean were found to have significant effects on androstenedione production by the Plackett-Burmandesignin shaker flask cultivation. The steepest ascent method was used to access the optimal region of the medium composition,followed by an application of response surface. The analysis revealed that the optimum values of thetested variables were 2% corn steep liquor,0. 07% Na H2PO4 and 14. 49% soybean oil. Under optimizedmedium,the average androstenedione production reached 6. 23 g·L- 1and the bioconversion rate was55. 3%,which was in agreement with the prediction. In comparison to the production of original level（ 4. 65 g·L- 1）,1. 34-fold increase had been obtained.
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2016年第3期239-244,共6页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
国家自然科学基金(31360217)
科技部农业科技成果转化项目
江西省科技支撑计划(2014BBG70016)资助项目
关键词
新金分支杆菌
雄烯二酮
耐底物
培养基优化
响应面
mycobacterium neoaurum MN4
4-androstene-3
17-dione
substrate-tolerant
medium optimization
response surface methodology
作者简介
通信作者：王筱兰（1965-），女，江西景德镇人，教授，博士，主要从事生物工艺原理、生物工程设备方面的研究．
