摘要
目的:基于Box-Behnken响应面法,采用双水相体系优化五味子多糖的提取工艺。方法:在单因素试验的基础上,选取药液量、K_2HPO_4用量、PEG6000用量为考察因素,以双水相体系的相比、分配系数和萃取效率的总评归一值(OD)为响应值,采用Box-Behnken响应面法筛选提取工艺。结果:最佳的五味子多糖双水相提取参数为:药液量5 m L,K_2HPO_4用量1.0 g,PEG6000用量1.8 g,离心时间9 min。该模型的预测性能良好,验证试验的实测OD值为0.950,与理论预测值的偏差为3.94%。结论:所优选的双水相提取技术操作简便、成本低,可用于五味子多糖的提取。
Objective： To optimize the extraction condition of polysaccharide from Schisandra chinensis. Aqueous two-phase extraction（ ATPE） method was used,based on Box-Behnken design with Response surface methodology（ BBD-RSM）. Methods： solvent volume,K_2HPO_4 and PEG6000 were selected as the investigation factors by the single-factor experiment,and the overall desirability（ OD）of phase volume ratio,partition coefficient and extraction rates were the reponse value. BBD-RSM was used to optimize the extraction process. Results： The optimal parameters were as follows,the solvent volume was 5 m L,the addition amount of K_2HPO_4 was 1. 0 g,the addition amount of PEG6000 was 1. 8 g and centrifugation time was 9 min,which indicated that the model had a good predictability. The predicted value was 0. 950,and the deviation between observed and predicted values was 3. 94%. Conclusion： The ATPE technology is easy to operate and cost-effective for the extraction of polysaccharides from Schisandra chinensis.
出处
《中药材》
CAS
CSCD
北大核心
2016年第3期593-597,共5页
Journal of Chinese Medicinal Materials
基金
国家中医药管理局中医药行业科研专项(201407003)
国家自然科学基金(81202903)
关键词
五味子
双水相体系
提取
多糖
响应面法
总评归一值
Schisandra chinensis（Turcz ） Baill ATPE Extraction Polysaccharide RSM OD value
作者简介
李化（1976-），女，博士，副研究员，主要从事中药化学与质量评价研究；E-mail：lihua1976@icmm．ac.cn
通讯作者：杨滨，Tel：010-64014411-2848，E-mail：ybinmm@126．com。
