摘要
实验以尾巨桉DH32-29(E.urophylla×E.grandis DH32-29)为实验材料,用沙培法研究外源磷(P)对铝胁迫下尾巨桉生长和生理方面的缓解作用。结果表明:(1)5 mmol·L^(-1)铝显著抑制尾巨桉的根、茎、叶生物量,显著提高根冠比。(2)铝胁迫抑制叶片叶绿素a、叶绿素b和类胡萝卜素质量分数,降低ω(叶绿素a)/ω(叶绿素b)值。(3)铝胁迫下叶片细胞膜透性显著增大,膜脂过氧化的产物丙二醛显著增加,细胞可溶性糖和可溶性蛋白质量分数显著降低,游离脯氨酸质量分数显著增大。(4)P缓解铝胁迫对光合色素的影响以及调节活性氧代谢以维持细胞膜结构稳定,有效缓解铝胁迫对尾巨桉生长、光合色素以及抗性生理造成的伤害,磷铝互作有利于缓解铝胁迫,P施用浓度为200μmol·L^(-1)时,缓解铝胁迫效果较好。
To verify the growth and physiological Feature of exogenous phosphorus on ameliorating aluminum toxicity in Eucalyptus,we planted E. urophylla×E. grandis DH32- 29 in sand solutions,and then detected the root and above-ground biomass,photosynthetic pigments,and antioxidant physiological indexes. Al of 5 mmol·L-1significantly inhibited the root and above-ground biomass and increased the root shoot ratio. Al of 5 mmol·L-1significantly inhibited the leaf Chl a / b and the contents of leaf Chl a,Chl b and Car. Al stress significantly increased cell membrane permeability and MDA（ the product of plasmalemma peroxidation） and free proline contents,decreased soluble sugar and soluble protein content in Eucalyptus leaf. Exogenous P played an important role in formation and inhibit decomposition of photosynthetic pigments,maintaining cell membrane structure stability by regulating reactive oxygen metabolism under Al toxicity,thereby reducing the Al damage to Eucalyptus growth photosynthetic pigments and resistance physiology. P of 200 μmol·L-1was better in the relieve effect under Al stress.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2016年第5期5-9,19,共6页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(31360174)
关键词
尾巨桉DH32-29
铝胁迫
磷
生长
生理
Eucalyptus urophylla×E
grandis DH32-29
Al stress
P
Growth
Physiological
作者简介
第一作者简介：侯文娟，女，1988年7月生，广西大学林学院，硕士研究生。E-mail：1083921118@qq．com。
通信作者：滕维超，广西大学林学院，讲师。E-mail：vincentt@yeah．net。
