摘要
目的:研究雷帕霉素对宫颈癌HeLa细胞侵袭转移的影响及其机制。方法:将HeLa细胞分为对照组和雷帕霉素低、中、高剂量(10、30、100 nmol/L)组,药物作用48 h后,MTT法检测细胞活力,计算抑制率;Transwell法检测细胞迁移和侵袭能力;Western blot法检测细胞中蛋白激酶B(Akt)、雷帕霉素靶蛋白(mTOR)磷酸化水平,以及基质金属蛋白酶2(MMP-2)、MMP-9、波形蛋白(Vimentin)、钙黏蛋白(E-cadherin)表达水平。结果:与对照组比较,雷帕霉素各剂量组细胞的抑制率增加(P<0.01),迁移细胞数目和侵袭细胞数目减少(P<0.01),MMP-2、MMP-9、Vimentin表达水平降低(P<0.01或P<0.05),E-cadherin表达水平增强(P<0.01或P<0.05),Akt及mTOR磷酸化水平降低(P<0.01)。结论:雷帕霉素可通过Akt/mTOR信号通路抑制He La细胞的侵袭转移。
OBJECTIVE：To study the effects and mechanism of rapamycin on invasion and metastasis of cervical cancer He La cell.METHODS：He La cells were divided into control group and rapamycin low-dose,medium-dose and high-dose groups（10,30,100 nmol/L）.After treated for 48 h,cell viability was measured by MTT assay,and inhibitory rate was calculated;migration and invasion of cell was tested by Transwell assay.The expression of matrix metalloproteinase 2（MMP-2）,MMP-9,Vimentin and E-cadherin,and phosphorylation of protein kinase B（Akt）,mammalian target of rapamycin（mTOR） were detected by Western blot.RESULTS：Compared with control group,the inhibition rate of cell viability was increased in rapamycin groups（P〈0.01）;the number of invasion and metastasis cells decreased（P〈0.01）;the expression of MMP-2,MMP-9 and Vimentin were decreased（P〈0.01 or P〈0.05）;the expression of E-cadherin was enhanced（P〈0.01 or P〈0.05）;the phosphorylation of Akt and mTOR were reduced（P〈0.01）.CONCLUSIONS：Rapamycin could inhibit invasion and metastasis of He La cell via Akt/mTOR signal pathway.
出处
《中国药房》
CAS
北大核心
2016年第16期2225-2228,共4页
China Pharmacy
作者简介
主治医师，硕士。研究方向：妇科肿瘤。电话：0311-85988742。E—mail：shjzjialigang@126．com
