摘要
利用超高效液相色谱-高分辨四极杆飞行时间质谱对预混剂中的黄霉素5种组分进行鉴别分析。采用C18色谱柱分离,以乙腈-0.1%甲酸水溶液(含2 mmol/L乙酸铵)为流动相梯度洗脱,流速0.4 m L/min,柱温40℃;质谱条件为电喷雾离子源,检测方式为负离子全扫描模式,通过保留时间、精确分子量和二级质谱特征碎片完成对黄霉素的5种组分的鉴别。与高效液相色谱方法比较,本方法具有判断准确、检测快速的特点,具有很好的应用价值。
Using ultra- performance liquid chromatography- tandem high resolution quadrupole time- of- flight mass spectrometry,the discriminant analysis of 5 components of flavomycin in premix was conducted. Based on C18 chromatogram column separation, the acetonitrile- 0. 1% formic acid aqueous solution（ including2 mmol / L ammonium acetate） was employed as the mobile phase at flow rate of 0. 4 mL / min for gradient elution at column temperature of 40 ℃; Mass spectrometry conditions were electrospray ionization（ ESI） operated in negative ion full scan mode. The identification of 5 components of flavomycin were implemented based on retention time,exact molecular weight, and secondary mass spectral characteristic fragments. Compared with high performance liquid chromatography,this method is of better application value for its advantages of accurate judgement and rapid detection.
出处
《中国兽药杂志》
北大核心
2016年第5期24-28,共5页
Chinese Journal of Veterinary Drug
基金
国家高技术研究发展计划(2011AA10A214)
关键词
鉴别
黄霉素
超高效液相色谱
高分辨四极杆飞行时间质谱
indentification
flavomycin
ultra-performance liquid chromatography
high resolution quadrupole time-of-flight mass spectrometry
作者简介
吴家鑫，高级工程师，从事兽药研发工作。E—mail：icebergwujiaxin@163．com
