摘要
目的:建立一种稳定的、可操作性强的病证结合大鼠帕金森病(PD)模型,以满足研究药物对PD治疗评价的需求。方法:采用6-羟基多巴胺脑部立体注射法构建PD动物模型,同时设立正常对照组、假手术组、模型组、药物(复方地黄方)组,从病因、症状、客观指标、药物反证等方面对PD大鼠模型进行综合判断,验证该模型的中医证候属性。结果:模型组大鼠的神经行为学、氧化应激、TH m RNA及蛋白、阳性细胞等指标与正常对照组比较,差异均有统计学意义(P<0.01)。药物组可明显改善大鼠的主观症状和上述客观指标,差异有统计学意义(P<0.01)。结论:6-羟基多巴胺大鼠PD模型的中医证候属性是风阳内动证。
Objective： To establish a steady and simplify combination of disease and syndrome rat model with Parkinson＇s disease（PD）, and meet the demand of drug study on treatment and evaluation of PD. Methods： The animal PD model was established by brain stereotaxic injection with 6-OHDA. The rats were divided into normal control group, sham operation group, model group and drug group. The PD model and its syndrome property were validated by a synthetic judgment from the aspects of etiology, symptoms, objective index, drugs counterevidence and so on. Results： The nerve etiology, oxidative stress, TH m RNA and protein and positive cell in model group were significantly different compared with the normal control group（P〈0.01）. Drug group could significantly improve the subjective symptoms and objective indexes of rats mentioned above, the difference was statistically significant（P〈0.01）. Conclusion： The TCM syndrome of PD 6-OHDA model rats was syndrome of internal stirring of wind yang.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2016年第5期1854-1857,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.81573899)
上海市科委新药开发研究项目(No.12401900302)
甘肃省科技厅课题(No.1010RJZA176)
甘肃省中医药管理局科研课题(No.GZK-2010-47)~~
关键词
病证结合
动物模型
帕金森病
大鼠
复方地黄方
Combination of disease and syndrome
Animal model
Parkinson
s disease
Rat
作者简介
通讯作者：何建成，上海市浦东新区蔡伦路1200号上海中医药大学基础医学院中医诊断教研室，邮编：201203电话（传真）：021-51322142，E-mail：hejc8163@163．com
