摘要
本文用神经毒药物6-OHDA注入大鼠右侧中脑被盖腹侧区，2周后用阿朴吗啡诱发旋转对受损动物进行筛选。结果发现：实验组125只大鼠中有52只鼠成为帕金森病模型，成功率为41．6％。用酪氨酸羟化酶(TH)抗体免疫组化法及荧光组化法对部分模型鼠的中脑及纹状体进行了组织学检查，证实受损侧黑质区等巴胺能神经元95%以上消失，纹状体TH阳性的黑质纹体纤维终末亦消失，但对侧存在。本实验为用脑移值治疗怕金森病的实验研究提供了可靠的动物模型。
Young adult Sprague-Dawley rats received unilateral injections of 8 μg/4 μl6-hydroxydopamine into the right nigrostriatal pathway. Two weeks after theinjections the animals were screened for the success of striatal denervation by atest of apomorphine-induced rotational behaviour. The rate of successful modelwas 41.6%. The fluorescence histochemistry and TH immunohistochemistryexamination revealed that reduction of neurons of right substantia nigra was more than 95%. However, neurons in the contralateral substantia nigra werenormal. This study provides reliable animal models for further experimentalresearch on therapy of Parkinson's disease with brain transplantation.
出处
《南通医学院学报》
1989年第2期84-86,T001,共4页
ACTA Academiae Medicinae Nantong
基金
国家自然科学基金资助课题的一部分
关键词
帕金森病
动物模型
黑质
中脑
纹状体
大鼠
治疗
发旋
用脑
消失
Parkinson's disease
model
rat
6-hydroxydopamine
tyrosine hydroxylase
immunohistochemistry assay
