摘要
本研究旨在探讨鸭I-FABP基因的结构与功能。本试验以绍兴麻鸭为研究材料,运用RACE、RT-PCR方法扩增鸭I-FABP基因mRNA全序列,运用qRT-PCR方法检测该基因在小肠不同区段的表达特性,运用siRNA干扰、qRT-PCR分析其功能。结果表明,鸭I-FABP基因共编码132个氨基酸,在不同物种间保守性较高,且在第60位氨基酸处发现一个Ile/Thr的突变位点;I-FABP在空肠前半段和十二指肠中表达量较高,与微粒体甘油三酯转移蛋白(Microsomal triglyceride transferprotein,MTTP)和载脂蛋白B(Apolipoprotein B,ApoB)基因在小肠不同肠段具有相似的表达变化情况,而与肝脏型脂肪酸结合蛋白基因(Liver fatty acid binding protein,L-FABP)存在差异;干扰I-FABP基因表达后,MTTP和ApoB基因的表达量显著降低,而L-FABP未发生显著变化。该结果预示了I-FABP基因在鸭的小肠脂肪酸吸收与转运通路中的重要作用,也为该基因的深入研究奠定了基础。
This study aimed to analyze the structure and function of duck I-FABP gene. The Sha- oxing duck was used. The sequence of duck I-FABP mRNA was cloned by RACE and RT-PCR. The expression patterns of I-FABP in different parts of small intestine were detected by qRT- PCR. The function was analyzed by siRNA and qRT-PCR. The duck I-FABP gene encodes a 132- amino acid protein. A mutation from T to C resulting in a substitution from Ile to Thr was found at codon 60 in exon 2 of the I-FABP. I-FABP,MTTP and ApoB mRNA had high expression lev- els in duodenum and the first half of jejunum and had slight differences with the expression of L-FABP. After the I-FABP interfered,the expression of MTTP and ApoB were decreased sig- nificantly, but no obvious change at the expression level of L-FABP. The results verify the impor- tant role of I-FABP in uptake and translocation of fatty acid in small intestine of ducks and pro- vide the foundation for further research.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2016年第5期1041-1048,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
湖北省创新团队项目(2016-620-000-001-028)
动物胚胎及分子育种湖北省重点实验室开放课题
湖北省农业科学院青年基金(2015NKYJJ27)
作者简介
陈芳（1987-），女，四川眉山人，助理研究员，博士，主要从事动物营养调控机理研究
